Baca-Regen L, Heinzinger N, Stevenson M, Gendelman H E
Department of Pathology, University of Nebraska Medical Center, Omaha 68198-5215.
J Virol. 1994 Nov;68(11):7559-65. doi: 10.1128/JVI.68.11.7559-7565.1994.
Alpha interferon (IFN-alpha) restricts multiple steps of the human immunodeficiency virus type 1 (HIV-1) life cycle. A well-described effect of IFN-alpha is in the modulation of viral nucleic acid synthesis. We demonstrate that IFN-alpha influences HIV-1 DNA synthesis principally by reducing the production of late products of reverse transcription. The magnitude of IFN-alpha-induced downregulation of HIV-1 DNA and/or progeny virion production was dependent on the IFN-alpha concentration, the duration of cytokine administration, the multiplicity of infection, the viral strain, and the cycles of viral infection. Interestingly, reductions in viral DNAs could not fully account for the observed IFN-alpha-induced abrogation of progeny virion production. These data, by our investigation of both single-cycle and spreading viral infections, support a predominant but not exclusive effect of IFN-alpha on viral DNA synthesis.
α干扰素（IFN-α）可限制人类免疫缺陷病毒1型（HIV-1）生命周期的多个步骤。IFN-α的一种广为人知的作用是调节病毒核酸合成。我们证明，IFN-α主要通过减少逆转录晚期产物的产生来影响HIV-1 DNA合成。IFN-α诱导的HIV-1 DNA下调和/或子代病毒体产生的程度取决于IFN-α浓度、细胞因子给药持续时间、感染复数、病毒株以及病毒感染周期。有趣的是，病毒DNA的减少并不能完全解释所观察到的IFN-α诱导的子代病毒体产生的消除。通过我们对单循环和传播性病毒感染的研究，这些数据支持IFN-α对病毒DNA合成具有主要但非排他性的作用。
