Yanagisawa H, Kurihara N, Klahr S, Morrissey J, Wada O
Department of Hygiene and Preventive Medicine, Faculty of Medicine, University of Tokyo, Japan.
Kidney Int. 1994 Jul;46(1):245-51. doi: 10.1038/ki.1994.266.
Dietary protein restriction ameliorates the decrease in GFR and renal plasma flow that occurs 24 hours after the onset of bilateral ureteral obstruction (BUO). The vasoactive hormones, prostaglandins (PGs) and thromboxane (Tx), have a role in the changes in renal function described above. Thus, we evaluated the effect of dietary protein restriction on the production of PGE2. 6-keto PGF1 alpha and TxB2 and on the activities of cyclooxygenase and phospholipases A2 and C in glomeruli isolated from sham-operated control (SOC) and BUO rats fed a low (6% casein) or a normal protein (23% casein) diet for approximately four weeks. A normal protein diet compared to a low protein diet significantly increased the glomerular production of PGE2, 6-keto PGF1 alpha and TxB2 in SOC rats. Glomeruli of rats with BUO fed a normal protein diet had further increased production of eicosanoids when compared to glomeruli of SOC rats ingesting the same diet. The production rates of eicosanoids correlated well with the activity of cyclooxygenase in the two groups of rats. On the other hand, a low protein diet completely abolished the increase in glomerular eicosanoid production seen in rats with BUO. The synthetic levels of eicosanoids were comparable in low protein-fed SOC and BUO rats, indicating normalization of glomerular eicosanoid production in BUO rats fed a low protein diet. Moreover, there were no significant differences in the activities of cyclooxygenase and phospholipases A2 and C between the SOC and BUO rats.(ABSTRACT TRUNCATED AT 250 WORDS)
饮食蛋白限制可改善双侧输尿管梗阻（BUO）发作24小时后出现的肾小球滤过率（GFR）和肾血浆流量的降低。血管活性激素、前列腺素（PGs）和血栓素（Tx）在上述肾功能变化中起作用。因此，我们评估了饮食蛋白限制对从假手术对照（SOC）和BUO大鼠分离的肾小球中PGE2、6-酮-PGF1α和TXB2的产生以及环氧化酶、磷脂酶A2和C活性的影响，这些大鼠分别喂食低蛋白（6%酪蛋白）或正常蛋白（23%酪蛋白）饮食约四周。与低蛋白饮食相比，正常蛋白饮食显著增加了SOC大鼠肾小球中PGE2、6-酮-PGF1α和TXB2的产生。与摄入相同饮食的SOC大鼠的肾小球相比，喂食正常蛋白饮食的BUO大鼠的肾小球类花生酸产生进一步增加。在两组大鼠中，类花生酸的产生率与环氧化酶的活性密切相关。另一方面，低蛋白饮食完全消除了BUO大鼠肾小球类花生酸产生的增加。低蛋白喂养的SOC和BUO大鼠中类花生酸的合成水平相当，表明喂食低蛋白饮食的BUO大鼠肾小球类花生酸产生正常化。此外，SOC和BUO大鼠之间环氧化酶、磷脂酶A2和C的活性没有显著差异。（摘要截断于250字）
