Sato K, Mano H, Ariyama T, Inazawa J, Yazaki Y, Hirai H
Third Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Japan.
Leukemia. 1994 Oct;8(10):1663-72.
Mouse Tec is a non-receptor type protein-tyrosine kinase and is highly expressed in many hematopoietic cell lines. To investigate the roles of the Tec kinase in the human hematopoietic system, we isolated cDNAs encoding the human Tec kinase. The human tec cDNAs can encode a peptide of 631 amino acid residues with a calculated molecular mass of 73,624. The predicted human Tec protein is highly homologous to those of the members of the Tec family including mouse Tec type IV (94% homology), mouse Tsk/Itk (60%), and human Btk (57%). The homology between human Tec and other members of the Tec family can be observed not only in the Src homology 3 (SH3), SH2, and kinase domains, but also in the N-terminal unique domain. Northern blot analysis demonstrated that the major transcripts of tec could be detected at 2.6 kb and 3.6 kb in a wide range of human hematopoietic cell lines including myeloid, B-, and T-cell lineages. Interestingly, high expression of the tec gene could be detected in all of the three patients examined with myelodysplastic syndrome. The human tec gene was mapped by fluorescence in situ hybridization (FISH) to chromosome 4p12.
小鼠Tec是一种非受体型蛋白酪氨酸激酶，在许多造血细胞系中高度表达。为了研究Tec激酶在人类造血系统中的作用，我们分离了编码人类Tec激酶的cDNA。人类tec cDNA可编码一个由631个氨基酸残基组成的肽段，计算分子量为73,624。预测的人类Tec蛋白与Tec家族成员高度同源，包括小鼠Tec IV型（94%同源性）、小鼠Tsk/Itk（60%）和人类Btk（57%）。人类Tec与Tec家族其他成员之间的同源性不仅可以在Src同源结构域3（SH3）、SH2和激酶结构域中观察到，在N端独特结构域中也能观察到。Northern印迹分析表明，在包括髓系、B细胞和T细胞系在内的多种人类造血细胞系中，可以检测到2.6 kb和3.6 kb的tec主要转录本。有趣的是，在所有三名骨髓增生异常综合征患者中都检测到了tec基因的高表达。通过荧光原位杂交（FISH）将人类tec基因定位到染色体4p12。
