López-Ribot J L, Navarro D, Sepúlveda P, Nogueira J M, Casanova M, Martinez J P
Department of Microbiology and Immunology, Texas Tech University Health Science Center, Lubbock 79430.
Mycopathologia. 1994 Jul;127(1):1-13. doi: 10.1007/BF01104005.
Characterization of common cell surface-bound antigens in Candida albicans strains, particularly those expressed in the walls of mycelial cells might be useful in the diagnosis of systemic candidiasis. Hence, antigenic similarities among wall proteins and mannoproteins from C. albicans clinical serotype A and B isolates, were studied using polyclonal (mPAbs) and monoclonal (MAb 4C12) antibodies raised against wall antigens from the mycelial form of a common C. albicans serotype A laboratory strain (ATCC 26555). Zymolyase digestion of walls isolated from cells of the different strains studied grown at 37 degrees C (germination conditions), released, in all cases, numerous protein and mannoprotein components larger than 100 kDa, along with a 33-34 kDa species. The pattern of major antigens exhibiting reactivity towards the mPAbs preparation was basically similar for all the serotype A and B isolates, though minor strain-specific bands were also observed. The immunodeterminant recognized by MAb 4C12 was found to be absent or present in very low amounts in C. albicans isolates other than the ATCC 26555 strain, yet high molecular weight species similar in size (e.g., 260 kDa) to the wall antigen against which MAb 4C12 was raised, were observed, particularly in wall digests from serotype A strains. Cell surface hydrophobicity, an apparently important virulence factor in C. albicans, of the cell population of each serotype B strain was lower than that of the corresponding serotype A counterparts, which is possibly due to the fact that the former strains exhibited a reduced ability to form mycelial filaments under the experimental conditions used.
白色念珠菌菌株中常见细胞表面结合抗原的特性分析，尤其是那些在菌丝体细胞壁中表达的抗原，可能有助于系统性念珠菌病的诊断。因此，我们使用针对常见的A型白色念珠菌实验室菌株（ATCC 26555）菌丝体形式的壁抗原产生的多克隆抗体（mPAbs）和单克隆抗体（MAb 4C12），研究了A型和B型白色念珠菌临床分离株壁蛋白和甘露糖蛋白之间的抗原相似性。对在37摄氏度（萌发条件）下生长的不同研究菌株的细胞分离得到的细胞壁进行溶菌酶消化，在所有情况下，均释放出大量大于100 kDa的蛋白质和甘露糖蛋白成分，以及一种33 - 34 kDa的成分。尽管也观察到了次要的菌株特异性条带，但所有A型和B型分离株对mPAbs制剂表现出反应性的主要抗原模式基本相似。发现除ATCC 26555菌株外，白色念珠菌分离株中不存在或仅以极低量存在被MAb 4C12识别的免疫决定簇，然而，观察到了与MAb 4C12所针对的壁抗原大小相似（例如260 kDa）的高分子量成分，特别是在A型菌株的壁消化物中。在每个B型菌株的细胞群体中，细胞表面疏水性（白色念珠菌中一个明显重要的毒力因子）低于相应的A型菌株，这可能是由于前一种菌株在所用实验条件下形成菌丝丝的能力降低所致。
