14-3-3蛋白对Raf-1的激活作用。
Activation of Raf-1 by 14-3-3 proteins.
作者信息
Fantl W J, Muslin A J, Kikuchi A, Martin J A, MacNicol A M, Gross R W, Williams L T
机构信息
Daiichi Research Center, Cardiovascular Research Institute, Department of Medicine, University of California, San Francisco 94143.
出版信息
Nature. 1994 Oct 13;371(6498):612-4. doi: 10.1038/371612a0.
The protein Raf-1, a key mediator of mitogenesis and differentiation, associates with p21ras (refs 1-3). However, the regulation of the serine/threonine kinase activity of Raf-1 is still not understood. Using the yeast two-hybrid system, we identified two structurally related proteins that interact with the aminoterminal region of Raf-1. These proteins, 14-3-3 zeta (PLA2) and 14-3-3 beta (HS1), are members of the 14-3-3 family of proteins. Expression of 14-3-3 proteins in Xenopus oocytes enhanced Raf-1 activity and promoted Raf-1-dependent oocyte maturation. A dominant negative mutant of Raf-1 blocked the effects of 14-3-3 protein.
蛋白质Raf-1是有丝分裂和分化的关键介质，与p21ras相关（参考文献1 - 3）。然而，Raf-1丝氨酸/苏氨酸激酶活性的调节仍不清楚。利用酵母双杂交系统，我们鉴定出两种与Raf-1氨基末端区域相互作用的结构相关蛋白。这些蛋白，14-3-3ζ（PLA2）和14-3-3β（HS1），是14-3-3蛋白家族的成员。14-3-3蛋白在非洲爪蟾卵母细胞中的表达增强了Raf-1活性，并促进了Raf-1依赖的卵母细胞成熟。Raf-1的显性负突变体阻断了14-3-3蛋白的作用。
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