Mukhopadhyay D, Anant S, Mukherji S
Department of Biophysics, Chittaranjan National Cancer Institute, Calcutta, India.
Neoplasma. 1994;41(3):151-7.
An inhibitor of poly(ADP-ribose) polymerase is 3-aminobenzamide (3AB), treatment by which normally results in the decreased levels of rejoining of DNA single strand breaks. We have studied the effect of 3AB in gamma-irradiation induced damage and the subsequent repair of DNA along with the corresponding changes in poly (ADP-ribose) polymerase activity in lymphocytes of patients with the cancer of breast and uterine cervix. The presence or absence of 3AB prior to gamma-irradiation did not influence the extent of DNA damage. On the other hand, single strand breaks rejoining in breast cancer cases was found to be slower as compared to that of cervical cancer cases and normal individuals. The relative ADP-ribosylation in the presence of 3AB was much lower in the breast cancer cases as compared to normal but there was no significant change in the cervical cancer cases. Overall the maximum relative ADP-ribosylation was slower in the presence of 3AB. This suggests that the lower activity of poly (ADP-ribose) polymerase in breast cancer cases might delay the first phase of single strand breaks (SSBs) rejoining.
聚（ADP - 核糖）聚合酶的一种抑制剂是3 - 氨基苯甲酰胺（3AB），用其处理通常会导致DNA单链断裂重连水平降低。我们研究了3AB对γ射线诱导的损伤以及随后DNA修复的影响，以及乳腺癌和宫颈癌患者淋巴细胞中聚（ADP - 核糖）聚合酶活性的相应变化。在γ射线照射之前是否存在3AB并不影响DNA损伤的程度。另一方面，发现乳腺癌病例中DNA单链断裂的重连比宫颈癌病例和正常个体要慢。与正常情况相比，在3AB存在的情况下，乳腺癌病例中的相对ADP - 核糖基化要低得多，但宫颈癌病例中没有显著变化。总体而言，在存在3AB的情况下，最大相对ADP - 核糖基化较慢。这表明乳腺癌病例中聚（ADP - 核糖）聚合酶的活性较低可能会延迟单链断裂（SSB）重连的第一阶段。
