Ahnström G, Ljungman M
University of Stockholm, Department of Radiobiology, Sweden.
Mutat Res. 1988 Jul;194(1):17-22. doi: 10.1016/0167-8817(88)90052-1.
The effect of 3-aminobenzamide (3AB), an inhibitor of poly(ADP-ribose) polymerase, on DNA-repair processes has been investigated after treating V79 hamster cells with methyl methanesulphonate (MMS). Repair activity was observed as changes in DNA-strand break levels. MMS induces transient strand breaks, the level of which slowly decreases with time. Addition of 3AB leads to a rapid increase in the number of breaks. The level of breaks increases linearly with time until it suddenly levels off. Increasing the concentration of 3AB does not change the slope of this curve, but the steady-state level of breaks increases. The incision-rejoining kinetics indicates that 3AB induces a delay in the strand-break rejoining process. In the absence of 3AB the breaks have a lifetime of 1-2 min and this is increased by a factor of 5 in the presence of 5 mM 3AB.
在用甲磺酸甲酯（MMS）处理V79仓鼠细胞后，研究了聚（ADP - 核糖）聚合酶抑制剂3 - 氨基苯甲酰胺（3AB）对DNA修复过程的影响。修复活性通过DNA链断裂水平的变化来观察。MMS诱导瞬时链断裂，其水平随时间缓慢下降。添加3AB会导致断裂数量迅速增加。断裂水平随时间呈线性增加，直到突然趋于平稳。增加3AB的浓度不会改变该曲线的斜率，但断裂的稳态水平会增加。切口 - 重新连接动力学表明3AB会导致链断裂重新连接过程延迟。在没有3AB的情况下，断裂的寿命为1 - 2分钟，在存在5 mM 3AB的情况下，寿命增加了5倍。
