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比色法微生物龋齿活性测试(Cariostat)在学龄前儿童中的敏感性和特异性。

The sensitivity and specificity of a colorimetric microbiological caries activity test (Cariostat) in preschool children.

作者信息

Koroluk L, Hoover J N, Komiyama K

机构信息

Division of Pediatric Dentistry, College of Dentistry, University of Saskatchewan, Saskatoon, Canada.

出版信息

Pediatr Dent. 1994 Jul-Aug;16(4):276-81.

PMID:7937260
Abstract

The purpose of this study was to test the ability of a colorimetric test, Cariostat (Sankin, Tokyo, Japan) to identify preschool children with dental caries. Three- to 5-year-old children (N = 153) from seven preschool programs in Saskatoon, Canada, were examined for dental caries using a mirror and explorer. A caries severity index (csi) was calculated for each subject. In all subjects buccal plaque samples were obtained, incubated, and scored as per the manufacturer's instructions for the Cariostat test. In 98 of 153 children whole stimulated saliva was also collected and population counts of Streptococcus mutans (CFU/ml of saliva) were determined. The test group exhibited mean dft = 1.03, mean dfs = 1.53, and mean csi = 0.46 while 66.67% of subjects were caries free and had no restorations. The S. mutans count was found to be significantly correlated (P < 0.001) with dft, dfs, and csi. Significant differences (P < 0.001) were found between Cariostat groups with respect to dft, dfs, and csi. The sensitivity of the Cariostat test was found to be 98% while the specificity was found to be 14%.

摘要

本研究的目的是测试比色试验Cariostat(日本东京参金公司)识别患有龋齿的学龄前儿童的能力。对来自加拿大萨斯卡通市七个学前教育机构的153名3至5岁儿童使用口镜和探针进行龋齿检查。为每个受试者计算龋齿严重程度指数(csi)。在所有受试者中,采集颊面菌斑样本,按照制造商对Cariostat试验的说明进行培养和评分。在153名儿童中的98名中,还收集了全唾液刺激样本,并测定了变形链球菌的菌落计数(每毫升唾液中的CFU)。试验组的平均龋失补牙数(dft)为1.03,平均龋补牙面数(dfs)为1.53,平均csi为0.46,同时66.67%的受试者无龋齿且无补牙。发现变形链球菌计数与dft、dfs和csi显著相关(P < 0.001)。在Cariostat试验组之间,在dft、dfs和csi方面发现了显著差异(P < 0.001)。发现Cariostat试验的敏感性为98%,而特异性为14%。

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