Chin M P, Smith C K, Li W I
Department of Physiology and Pharmacology, College of Veterinary Medicine, University of Georgia, Athens 30602.
Peptides. 1994;15(4):645-50. doi: 10.1016/0196-9781(94)90089-2.
Incubation of a rabbit endometrial cell line (HRE-H9 cells) with KCl (5-60 mM) for 30 min enhanced IR-GnRH secretion, with 30 and 60 mM producing the greatest stimulatory effect (280 +/- 19% and 298 +/- 49% of control group, respectively). By adding 30 mM KCl into HRE-H9 culture and increasing the incubation time to 90 min, there was a stepwise increase in IR-GnRH secretion. In the third experiment, treatment of HRE-H9 cells with estradiol (E2, 10(-9)-10(-8) M) for 48 h stimulated IR-GnRH secretion (215 +/- 17%, 168 +/- 19%, respectively), whereas P4 treatment did not produce any significant change. Treatment with E2 + P4 at all doses tested (10(-10)-10(-6) M) augmented the secretion of IR-GnRH (140 +/- 16%, 153 +/- 14%, 276 +/- 23%, 259 +/- 26%, 198 +/- 16%, respectively). Increased IR-GnRH secretion by E2 (10(-9) M) and E2 + P4 (10(-8)-10(-7) M) resulted in a reduction in cell content of IR-GnRH (p < 0.05). In conclusion, secretion of IR-GnRH by HRE-H9 cells can be induced by KCl depolarization. Treatment of HRE-H9 cells with E2 and E2 + P4 enhanced their secretion of IR-GnRH. Under such conditions, secreted IR-GnRH appears to be derived primarily from intracellular IR-GnRH pools.
用氯化钾（5 - 60 mM）孵育兔子宫内膜细胞系（HRE - H9细胞）30分钟可增强免疫反应性促性腺激素释放激素（IR - GnRH）的分泌，其中30 mM和60 mM产生的刺激作用最大（分别为对照组的280±19%和298±49%）。向HRE - H9细胞培养物中添加30 mM氯化钾并将孵育时间延长至90分钟，IR - GnRH的分泌呈逐步增加。在第三个实验中，用雌二醇（E2，10⁻⁹ - 10⁻⁸ M）处理HRE - H9细胞48小时可刺激IR - GnRH的分泌（分别为215±17%，168±19%），而孕酮（P4）处理未产生任何显著变化。在所有测试剂量（10⁻¹⁰ - 10⁻⁶ M）下用E2 + P4处理可增加IR - GnRH的分泌（分别为140±16%，153±14%，276±23%，259±26%，198±16%）。E2（10⁻⁹ M）和E2 + P4（10⁻⁸ - 10⁻⁷ M）使IR - GnRH分泌增加导致细胞内IR - GnRH含量降低（p < 0.05）。总之，HRE - H9细胞分泌IR - GnRH可被氯化钾去极化诱导。用E2和E2 + P4处理HRE - H9细胞可增强其IR - GnRH的分泌。在这种情况下，分泌的IR - GnRH似乎主要来源于细胞内的IR - GnRH库。
