Rubinstein A, Tirosh B
David R. Bloom Center for Pharmacy, School of Pharmacy, Hebrew University of Jerusalem, Israel.
Pharm Res. 1994 Jun;11(6):794-9. doi: 10.1023/a:1018961204325.
The thickness of the mucus gel and its turnover rate were measured in the stomach, proximal jejunum, cecum and proximal colon of the rat, using microscopy and staining techniques. The specific mucus-secretory responses to carbachol-induced cholinergic stimulus in these locations were also studied. The mucus gel was found to be the thinnest (18 +/- 1 microns) in the cecum, and the thickest in the stomach (39 +/- 14 microns). The effect of carbachol on mucus secretion was profound and dose dependent in the stomach, and less profound, although still dose dependent, in the proximal jejunum. The least responsive organs were the cecum and the proximal colon, where no effect was observed after three doses of carbachol. Mucus secretion rate was significantly higher in the jejunum (1.1 +/- 0.5 microgram glucose equivalent min-1 cm-2) than in the colon (0.5 +/- 0.2 microgram glucose equivalent min-1 cm-2). Also, the proximal jejunum was more responsive to the carbachol stimulus (mucus secretion rate of 5.4 +/- 2.2 micrograms glucose equivalent min-1 cm-2 after carbachol treatment) than the colon (mucus secretion rate of 1.0 +/- 0.4 micrograms glucose equivalent min-1 cm-2 after carbachol treatment). In vitro mucoadhesion studies with Polycarbophil disks were performed in the mucosal tissues of the stomach, jejunum, cecum and proximal colon of the rat with and without cholinergic (carbachol) stimulus. The adhesion force in the cecum and the colon was significantly stronger than in the stomach and proximal jejunum when the studies were performed at pH 2.(ABSTRACT TRUNCATED AT 250 WORDS)
采用显微镜检查和染色技术，测量了大鼠胃、空肠近端、盲肠和结肠近端黏液凝胶的厚度及其更新率。还研究了这些部位对卡巴胆碱诱导的胆碱能刺激的特异性黏液分泌反应。结果发现，盲肠中的黏液凝胶最薄（18±1微米），胃中最厚（39±14微米）。卡巴胆碱对胃黏液分泌的影响显著且呈剂量依赖性，在空肠近端影响较小，虽仍呈剂量依赖性。反应最弱的器官是盲肠和结肠近端，给予三剂卡巴胆碱后未观察到影响。空肠中的黏液分泌率（1.1±0.5微克葡萄糖当量·分钟⁻¹·厘米⁻²）显著高于结肠（0.5±0.2微克葡萄糖当量·分钟⁻¹·厘米⁻²）。此外，空肠近端对卡巴胆碱刺激的反应性（卡巴胆碱处理后黏液分泌率为5.4±2.2微克葡萄糖当量·分钟⁻¹·厘米⁻²）高于结肠（卡巴胆碱处理后黏液分泌率为1.0±0.4微克葡萄糖当量·分钟⁻¹·厘米⁻²）。使用聚卡波非圆盘在有或无胆碱能（卡巴胆碱）刺激的情况下，对大鼠胃、空肠、盲肠和结肠近端的黏膜组织进行了体外黏膜黏附研究。当在pH 2条件下进行研究时，盲肠和结肠中的黏附力明显强于胃和空肠近端。（摘要截选至250字）
