胆汁淤积性婴儿肝脏中的胆盐磺基转移酶活性

Bile salt sulphotransferase activity in the liver of cholestatic infants.

作者信息

Obinata K, Nemeth A, Ellin A, Strandvik B

机构信息

Department of Paediatrics, Karolinska Institutet, Huddinge, Hospital, Stockholm, Sweden.

出版信息

Scand J Clin Lab Invest. 1994 Jul;54(4):285-90. doi: 10.3109/00365519409087524.

DOI:10.3109/00365519409087524

PMID:7939371

Abstract

A simple assay of bile salt sulphotransferase activity in human liver was developed. The system used glycolithocholate and PAPS as substrates. Km values for glycolithocholate and PAPs were 2.8 microM and 11.5 microM, respectively. Furthermore bile salt sulphation capacity in infants with cholestasis was investigated by measuring the activity of the bile salt sulphotransferase in the liver. No significant difference was found between the sulphotransferase activity in cholestatic infants and non-cholestatic adults. In addition the magnitude of the bile salt sulphotransferase activity in as neonatal liver did not differ from the enzymatic activity in adult liver. It is thus considered unlikely that low degree of sulphation of bile salts in infants is due to reduced capacity of this enzyme system.

摘要

开发了一种简单的人肝脏胆汁盐磺基转移酶活性测定方法。该系统使用甘氨石胆酸和PAPS作为底物。甘氨石胆酸和PAPS的Km值分别为2.8微摩尔和11.5微摩尔。此外，通过测量肝脏中胆汁盐磺基转移酶的活性，研究了胆汁淤积婴儿的胆汁盐硫酸化能力。胆汁淤积婴儿和非胆汁淤积成人的磺基转移酶活性之间未发现显著差异。此外，新生儿肝脏中胆汁盐磺基转移酶活性的大小与成人肝脏中的酶活性没有差异。因此，认为婴儿胆汁盐硫酸化程度低不太可能是由于该酶系统能力降低所致。