对ors 8（一种富含哺乳动物起源序列）的体外复制产物进行电子显微镜分析。

Electron microscopic analysis of in vitro replication products of ors 8, a mammalian origin enriched sequence.

作者信息

Pearson C E, Shihab-el-Deen A, Price G B, Zannis-Hadjopoulos M

机构信息

McGill Cancer Centre, Department of Medicine, McGill University, Montreal, Quebec, Canada.

出版信息

Somat Cell Mol Genet. 1994 May;20(3):147-52. doi: 10.1007/BF02254755.

DOI:10.1007/BF02254755

PMID:7940016

Abstract

Electron microscopy was used to map the initiation site of ors 8 DNA replication in vitro in a system that is capable of initiating and supporting one round of semiconservative replication of cloned mammalian DNA origin-enriched sequences (ors). Using unique restriction sites in ors 8 plasmid DNA, we have mapped the replication bubble within the monkey DNA sequence. In addition to site-specific initiation within the ors, the results also indicate bidirectional replication.

摘要

在一个能够起始并支持一轮克隆的富含哺乳动物DNA复制起点序列（ors）的半保留复制的系统中，利用电子显微镜对体外ors 8 DNA复制的起始位点进行了定位。通过ors 8质粒DNA中的独特限制性位点，我们在猴DNA序列内定位了复制泡。除了在ors内的位点特异性起始外，结果还表明存在双向复制。