Robledo M, Martínez B, Arranz E, Marcos B, Rivas C, Benítez J
Fundación Jiménez Díaz, Departamento de Genética, Madrid.
Sangre (Barc). 1994 Jun;39(3):187-9.
To evaluate the reliability of a PCR technique for the detection of monoclonal B-cell non-Hodgkin's lymphoma (NHL) in a group of patients previously showing monoclonal gene rearrangement with Southern Blot techniques.
A group of 22 cases of NHL were studied with immunocytochemical techniques by means of a complete monoclonal antibody panel after previous demonstration of monoclonal rearrangement with the JH probe. Specific Fr2 and Fr3 priming of the variable regions of the IgH genes was used. One positive and two negative controls were used on each PCR test.
Monoclonal lymphoma was detected in 18 cases (82%), seven of them with Fr2 and Fr3, whereas five cases had Fr2 and the remainders had only Fr3.
PCR seems a good alternative for detecting monoclonal lymphoproliferative syndromes instead of Southern Blot due to both its specificity and high sensitivity. The hypotheses to explain false negative results are discussed.
在一组先前通过Southern印迹技术显示单克隆基因重排的患者中，评估一种用于检测单克隆B细胞非霍奇金淋巴瘤（NHL）的PCR技术的可靠性。
在先前用JH探针证实单克隆重排后，通过完整的单克隆抗体组采用免疫细胞化学技术对一组22例NHL患者进行研究。使用针对IgH基因可变区的特异性Fr2和Fr3引物。每次PCR检测使用一个阳性对照和两个阴性对照。
在18例（82%）中检测到单克隆淋巴瘤，其中7例同时具有Fr2和Fr3，5例具有Fr2，其余仅具有Fr3。
由于其特异性和高灵敏度，PCR似乎是检测单克隆淋巴增殖综合征而非Southern印迹的良好替代方法。讨论了解释假阴性结果的假设。
