Mosesso P, Turchi G, Cinelli S, Di Chiara D, Fiore M, Palitti F
Università della Tuscia, Dipartimento di Agrobiologia e Agrochimica, Viterbo, Italy.
Teratog Carcinog Mutagen. 1994;14(3):145-55. doi: 10.1002/tcm.1770140306.
We report here the results obtained using the dithiocarbamate fungicides thiram and ziram to investigate the induction of chromosomal aberrations (CAs) in Chinese hamster ovary (CHO) cells both in the absence and presence of S9 metabolism, and in a Chinese hamster epithelial liver (CHEL) cells which retain metabolic competence to activate different classes of promutagens/procarcinogens. Both thiram and ziram proved to be strong chromosome breaking agents in the CHEL cells and CHO cells in the presence of S9 metabolism. These findings suggest that thiram and ziram require metabolic conversion to become genetically active, and corroborate the evidence that CHEL cells are suitable to activate and detect a broad spectrum of chemical procarcinogens including these two pesticides.
我们在此报告使用二硫代氨基甲酸盐类杀菌剂福美双和代森锌所获得的结果，以研究在有无S9代谢的情况下，它们对中国仓鼠卵巢（CHO）细胞染色体畸变（CA）的诱导作用，以及在保留激活不同类别的前诱变剂/前致癌物代谢能力的中国仓鼠肝上皮（CHEL）细胞中的作用。在有S9代谢的情况下，福美双和代森锌在CHEL细胞和CHO细胞中均被证明是强染色体断裂剂。这些发现表明，福美双和代森锌需要代谢转化才能具有遗传活性，并证实了CHEL细胞适合激活和检测包括这两种农药在内的广泛化学前致癌物的证据。
