2'-(3α-苄氧基-24-降胆烷-23-基)-2',4',4'-三甲基-4',5'-二氢恶唑啉-N-氧基作为模型膜的潜在自旋探针。

2'-(3 alpha-Benzyloxy-24-norcholan-23-yl)-2',4',4'-trimethyl-4',5'- dihydrooxazoline-N-oxyl as a potential spin probe for model membranes.

作者信息

Banerjee S, Trivedi G K, Srivastava S, Phadke R S

机构信息

Department of Chemistry, Indian Institute of Technology, Bombay.

出版信息

Steroids. 1994 Jun;59(6):377-82. doi: 10.1016/0039-128x(94)90005-1.

DOI:10.1016/0039-128x(94)90005-1

PMID:7940616

Abstract

A new steroidal doxyl (4,4-dimethyloxazolidine-N-oxyl) nitroxide (SDN) viz. 2'-(3 alpha-benzyloxy-24-norcholan-2'-yl)-2',4',4'-trimethyl-4',5'- dihydrooxazoline-N-oxyl has been synthesized. This is expected to have higher mobility over other spin labels reported earlier. The localization of this spin probe in lipid bilayers has been determined using 1H NMR and 31P NMR techniques. The alterations induced by drugs in the membrane characteristics such as phase transition and permeability have been investigated using electron paramagnetic resonance (EPR) techniques. The results show the applicability of SDN as a potential spin probe in the study of biomembranes.

摘要

一种新的甾体二氧自由基（4,4-二甲基恶唑啉-N-氧自由基）（SDN），即2'-（3α-苄氧基-24-降胆烷-2'-基）-2'，4'，4'-三甲基-4'，5'-二氢恶唑啉-N-氧自由基已被合成。预计它比先前报道的其他自旋标记物具有更高的流动性。已使用1H NMR和31P NMR技术确定了这种自旋探针在脂质双层中的定位。已使用电子顺磁共振（EPR）技术研究了药物引起的膜特性变化，如相变和通透性。结果表明SDN作为一种潜在的自旋探针在生物膜研究中的适用性。