Fields S, Sternglanz R
Department of Molecular Genetics and Microbiology, State University of New York at Stony Brook 11794.
Trends Genet. 1994 Aug;10(8):286-92. doi: 10.1016/0168-9525(90)90012-u.
The two-hybrid system is a yeast-based genetic assay for detecting protein-protein interactions. It can be used to identify proteins that bind to a protein of interest, or to delineate domains or residues critical for an interaction. Variations on this methodology have been developed to clone genes that encode DNA-binding proteins, to identify peptides that bind to a protein and, potentially, to screen for drugs.
双杂交系统是一种基于酵母的用于检测蛋白质-蛋白质相互作用的遗传学分析方法。它可用于鉴定与感兴趣的蛋白质结合的蛋白质，或描绘对相互作用至关重要的结构域或残基。已开发出该方法的变体，用于克隆编码DNA结合蛋白的基因、鉴定与蛋白质结合的肽，以及潜在地用于筛选药物。
