Lautincik J, Kolodzieyski L, Elias V, Hyttel P, Osawa Y, Sirotkin A
Department of Reproduction and Embryology, Veterinary University, Kosice, Slovak Republic.
Acta Vet Scand. 1994;35(2):185-91. doi: 10.1186/BF03548346.
The localization of aromatase, an enzyme converting androgens to estrogen, in the ovaries of superovulated cattle, pigs and sheep was studied immunocytochemically in the preovulatory and postovulatory period using anti-human placental aromatase cytochrome P-450 antiserum. Immunostaining for aromatase was detected in the granulosa cells of preovulatory follicles of all species studied. Theca interna cells were stained in preovulatory follicles in the pig but not in cattle and sheep. Interstitial gland cells, cumulus cells and oocytes were unstained in all species. In cattle and pig the corpora lutea were unstained whereas they displayed staining in the sheep. Preantral and small antral follicles were unstained during both the preovulatory and postovulatory period in all species. It is concluded that granulosa cells of preovulatory follicles are the main residence for aromatase activity in superovulated cattle, pig and sheep, whereas the activity of theca interna and corpora lutea is species specific.
利用抗人胎盘芳香化酶细胞色素P - 450抗血清，对超数排卵的牛、猪和羊卵巢中芳香化酶（一种将雄激素转化为雌激素的酶）在排卵前和排卵后的定位进行了免疫细胞化学研究。在所研究的所有物种的排卵前卵泡的颗粒细胞中均检测到芳香化酶的免疫染色。猪排卵前卵泡的内膜细胞有染色，但牛和羊没有。间质腺细胞、卵丘细胞和卵母细胞在所有物种中均无染色。牛和猪的黄体无染色，而在羊中黄体有染色。在所有物种的排卵前和排卵后时期，初级卵泡和小窦状卵泡均无染色。得出的结论是，在超数排卵的牛、猪和羊中，排卵前卵泡的颗粒细胞是芳香化酶活性的主要所在部位，而内膜细胞和黄体的活性具有物种特异性。
