Laurincik J, Oberfranc M, Hyttel P, Grafenau P, Tomanek M, Pivko J
Research Institute of Animal Production Department of Reproduction and Embryology Hlohovská 2, 949 42 Nitra, Czechoslovakia.
Theriogenology. 1993 Feb;39(2):537-44. doi: 10.1016/0093-691x(93)90395-l.
Heifers (n=31) were superovulated with an FSH-P/cloprostenol regimen, and at 12 and 24 hours after the onset of estrus they were inseminated. Blood sampling for LH analyses and ultrasound scanning of the ovaries were performed at 4-hours intervals. The scanning, at which the first and last ovulations were recorded, was performed at 22.7 +/- 1.5 (mean +/- SD) and 31.0 +/- 1.5 hours after the LH peak, respectively. An average of 7.8 +/- 1.0 ovulations was monitored when the first ovulations were detected, while 2.8 +/- 0.7 ovulations occurred later. At 16 hours after detection of the first ovulations the oviducts were flushed and 5.6 +/- 0.5 fertilized and 2.3 +/- 0.3 unfertilized ova were isolated per animal. The fertilized ova displayed spherical pronuclei of synchronous development, and polyspermic penetration was not seen. At 24 hours after detection of the first ovulations the content of the remaining 3.3 +/- 0.5 nonovulatory follicles > 8 mm per animal was aspirated. Expanded cumulus investment was found in 69.4% of the oocytes, while 22.4% had abstricted the first polar body.
对31头小母牛采用促卵泡素-P/氯前列醇方案进行超数排卵处理，在发情开始后的12小时和24小时进行输精。每隔4小时采集血液样本进行促黄体生成素（LH）分析，并对卵巢进行超声扫描。分别在LH峰值后22.7±1.5（平均值±标准差）小时和31.0±1.5小时进行扫描，记录首次排卵和末次排卵情况。检测到首次排卵时，平均监测到7.8±1.0个排卵，而之后发生2.8±0.7个排卵。在检测到首次排卵后16小时冲洗输卵管，每头动物分离出5.6±0.5个受精卵和2.3±0.3个未受精卵。受精卵呈现同步发育的球形原核，未见多精受精现象。在检测到首次排卵后24小时，抽吸每头动物剩余的3.3±0.5个直径大于8mm的未排卵卵泡内容物。发现69.4%的卵母细胞有扩展的卵丘包裹，22.4%的卵母细胞排出了第一极体。
