Miquel J F, Von Ritter C, Del Pozo R, Lange V, Jüngst D, Paumgartner G
Department of Medicine II, Klinikum Grosshadern, Ludwig-Maximilians-University, Munich, Germany.
Am J Physiol. 1994 Sep;267(3 Pt 1):G393-400. doi: 10.1152/ajpgi.1994.267.3.G393.
Some biliary proteins (pronucleators) seem to be essential factors for cholesterol crystal formation and crystal growth in bile. A recent study suggests that fibronectin is such a pronucleator in bile. Fibronectin also seems to closely interact with intestinal mucin. Since biliary mucin plays an important role in gallstone formation, such an interaction in bile may be of relevance in cholesterol gallstone formation. To more clearly elucidate the role of fibronectin in cholesterol gallstone disease, we measured the concentration of fibronectin in native bile of cholesterol gallstone patients and checked its influence on the cholesterol nucleation time of model bile. We further looked for a molecular interaction between biliary fibronectin and gallbladder mucin. We found that fibronectin is present in gallbladder bile of gallstone patients in low concentrations (2.6 +/- 1.2 micrograms/ml). Bile fibronectin did not interact with gallbladder mucin. Moreover, in a wide range of concentrations fibronectin had no influence on the nucleation time of model bile. We conclude that fibronectin does not seem to play a major role in cholesterol gallstone disease.
一些胆汁蛋白（成核前体）似乎是胆汁中胆固醇晶体形成和晶体生长的关键因素。最近一项研究表明，纤连蛋白就是胆汁中的这样一种成核前体。纤连蛋白似乎还与肠道黏蛋白密切相互作用。由于胆汁黏蛋白在胆结石形成中起重要作用，胆汁中的这种相互作用可能与胆固醇胆结石的形成有关。为了更清楚地阐明纤连蛋白在胆固醇胆结石疾病中的作用，我们测量了胆固醇胆结石患者天然胆汁中纤连蛋白的浓度，并检测了其对模型胆汁胆固醇成核时间的影响。我们还进一步探寻了胆汁纤连蛋白与胆囊黏蛋白之间的分子相互作用。我们发现，纤连蛋白以低浓度（2.6±1.2微克/毫升）存在于胆结石患者的胆囊胆汁中。胆汁纤连蛋白不与胆囊黏蛋白相互作用。此外，在很宽的浓度范围内，纤连蛋白对模型胆汁的成核时间没有影响。我们得出结论，纤连蛋白似乎在胆固醇胆结石疾病中不发挥主要作用。
