Green D F, Yates J A
Department of Biological Sciences, Oakland University, Rochester, Michigan.
Am J Trop Med Hyg. 1994 Oct;51(4):483-8.
Over the past several decades, epidemiologic data from filarial vectors typically has been obtained by mass dissection or by dissection of individual specimens. The former is quick and easy to do on large numbers of insects but provides no information on the frequency distribution of infection, presence of early developmental stages, or larval location; the latter is labor-intensive and tedious. We describe a new technique that can provide data comparable to those obtained by individual dissection, including calculation of infection and infective rates, and this technique is easy enough to accommodate large numbers of insects. Brief treatment of ethanol-fixed, intact mosquitoes in sodium hypochlorite, followed by treatments in increasing concentrations of ethanol and an organic solvent allowed microscopic visualization of filarial larvae within the abdomen, thorax, head, and proboscis of Brugia malayi-infected Aedes aegypti and Wuchereria bancrofti-infected Anopheles punctulatus. We compared the classic techniques to our technique using Ae. aegypti infected by feeding on jirds with B. malayi microfilaremias. Comparisons of the infective rate, total number of infective stage larvae (L3s) observed, and locations of L3s showed that this new technique was comparable to the established methods, while being faster and more precise in determining the location of larvae.
在过去几十年中，来自丝虫病媒介的流行病学数据通常是通过大规模解剖或单个标本解剖获得的。前者对大量昆虫进行操作快速简便，但无法提供感染频率分布、早期发育阶段的存在情况或幼虫位置的信息；后者则 labor-intensive 且繁琐。我们描述了一种新技术，它能够提供与通过单个解剖获得的数据相当的数据，包括感染率和感染率的计算，并且该技术足够简便，能够处理大量昆虫。将用乙醇固定的完整蚊子在次氯酸钠中进行简短处理，随后在浓度递增的乙醇和一种有机溶剂中进行处理，使得在显微镜下能够观察到感染马来布鲁线虫的埃及伊蚊以及感染班氏吴策线虫的微小按蚊腹部、胸部、头部和喙部内的丝虫幼虫。我们使用通过取食患有马来布鲁线虫微丝蚴血症的沙鼠而感染的埃及伊蚊，将经典技术与我们的技术进行了比较。对感染率、观察到的感染期幼虫（L3）总数以及 L3 的位置进行比较后发现，这种新技术与既定方法相当，同时在确定幼虫位置方面更快且更精确。
