Bell K
Department of Physiology and Pharmacology, University of Queensland, St Lucia, Brisbane, Australia.
Anim Genet. 1994 Jun;25 Suppl 1:109-13. doi: 10.1111/j.1365-2052.1994.tb00413.x.
Transferrin, albumin, 6-phosphogluconate dehydrogenase and vitamin D-binding protein polymorphisms were detected in 242 feral and domesticated Australian donkeys by polyacrylamide gel electrophoresis, starch gel electrophoresis, autoradiography, immunoblotting with specific antisera and activity staining. All four TF and two ALB variants were donkey specific while only one of the PGD variants was donkey specific. The two GC variants were electrophoretically identical to the Equus caballus F and S proteins. Available evidence suggested that the TF, ALB, PGD and GC systems are controlled by co-dominant alleles with frequencies of the most common alleles of each system being 0.831, 0.946, 0.957 and 0.861 respectively. Glucose phosphate isomerase and plasminogen were monomorphic in the Australian population of donkeys.
通过聚丙烯酰胺凝胶电泳、淀粉凝胶电泳、放射自显影、用特异性抗血清进行免疫印迹以及活性染色,在242头野生和家养的澳大利亚驴中检测了转铁蛋白、白蛋白、6-磷酸葡萄糖酸脱氢酶和维生素D结合蛋白的多态性。所有四种转铁蛋白(TF)变体和两种白蛋白(ALB)变体是驴特有的,而6-磷酸葡萄糖酸脱氢酶(PGD)变体中只有一种是驴特有的。两种维生素D结合蛋白(GC)变体在电泳上与马的F和S蛋白相同。现有证据表明,TF、ALB、PGD和GC系统由共显性等位基因控制,每个系统最常见等位基因的频率分别为0.831、0.946、0.957和0.861。磷酸葡萄糖异构酶和纤溶酶原在澳大利亚驴种群中是单态的。