Dubey J P, Briscoe N, Gamble R, Zarlenga D, Humphreys J G, Thulliez P
Zoonotic Diseases Laboratory, Agricultural Research Service, Beltsville, MD 20705-2350.
Am J Vet Res. 1994 Jun;55(6):815-9.
During the hunting season of 1992, 322 black bears from Pennsylvania were examined for Toxoplasma gondii- and Trichinella spp-induced infections. Toxoplasma gondii antibodies were found in 79.8% of 322 bears--titer < 1:25 in 65 (20.2%), 1:25 in 18 (5.6%), 1:50 in 11 (34.5%) and 1:500 in 128 (38.7%) bears--by use of the modified agglutination test. Muscle tissues from 89 of these bears were bioassayed for T gondii parasites. Muscles from 64 bears, including heart from 1 bear, and heart alone from another bear, were digested in pepsin, and the digested samples were bioassayed in mice. Toxoplasma gondii was isolated from 5 bears; from the heart of 1, heart and skeletal muscles of 1, and skeletal muscles of 3. The T gondii antibody titers for the 5 bears with detectable T gondii were: > or = 1:25 in all 5 bears by use of the modified agglutination test; < 1:10 (3 bears, considered Toxoplasma-negative), 1:20 and 1:320 by use of the Sabin-Feldman dye test; < 1:64 (3 bears, considered Toxoplasma-negative), 1:128, 1:512 by use of the indirect hemagglutination test, and < 1:16 (2 bears, considered Toxoplasma-negative), 1:32, 1:64, and 1:512 by use of the latex agglutination test. Toxoplasma gondii was not isolated from feces of 5 cats fed muscles from the remaining 25 bears with T gondii antibody titer < 1:25. Tissue cysts of the 4 T gondii isolates from bears were rendered noninfective by freezing at -13 C.(ABSTRACT TRUNCATED AT 250 WORDS)
在1992年的狩猎季节，对宾夕法尼亚州的322头黑熊进行了检查，以检测弓形虫和旋毛虫属引发的感染情况。通过改良凝集试验发现，322头熊中有79.8%存在弓形虫抗体——65头（20.2%）熊的滴度<1:25，18头（5.6%）熊的滴度为1:25，11头（34.5%）熊的滴度为1:50，128头（38.7%）熊的滴度为1:500。对其中89头熊的肌肉组织进行了弓形虫寄生虫的生物测定。64头熊的肌肉，包括1头熊的心脏以及另一头熊单独的心脏，用胃蛋白酶进行消化，消化后的样本在小鼠身上进行生物测定。从5头熊身上分离出了弓形虫；分别来自1头熊的心脏、1头熊的心脏和骨骼肌以及3头熊的骨骼肌。对5头检测到弓形虫的熊进行的弓形虫抗体滴度检测结果如下：通过改良凝集试验，所有5头熊的滴度≥1:25；通过Sabin-Feldman染色试验，3头熊（被视为弓形虫阴性）的滴度<1:10，1头熊的滴度为1:20，1头熊的滴度为1:320；通过间接血凝试验，3头熊（被视为弓形虫阴性）的滴度<1:64，1头熊的滴度为1:128，1头熊的滴度为1:512；通过乳胶凝集试验，2头熊（被视为弓形虫阴性）的滴度<1:16，1头熊的滴度为1:32，1头熊的滴度为1:64，1头熊的滴度为1:512。从其余25头弓形虫抗体滴度<1:25的熊身上获取肌肉喂食5只猫，在这些猫的粪便中未分离出弓形虫。将从熊身上分离出的4株弓形虫的组织囊肿在-13℃下冷冻，使其失去感染性。（摘要截选至250字）
