Giam Y C, Ong B H
National Skin Centre, Singapore.
Ann Acad Med Singap. 1994 May;23(3):412-7.
The study utilised monoclonal antibodies for T and B cells to study the immunophenotype in our lymphomas and to correlate with the clinicopathological aspects of eight cases of malignant lymphomas, of which four are cutaneous T cell lymphoma and four malignant lymphomas. The method used was immunohistochemical staining of tissue section by the alkaline phosphatase anti-alkaline phosphatase (APAAP) procedure. For four cases of cutaneous T cell lymphoma, three were in stage 1 and one in stage 2. The immunophenotypes were typical of mycosis fungoides-type cutaneous T cell lymphoma. They were predominantly T cells with many mature T cells. The CD4 to CD8 ratio fell within the normal range. There was no specific loss of CD7. For four cases of malignant lymphoma, T cell type, the pattern was again T cell phenotype with all of CD2, CD5, CD7 and CD4. The CD4 to CD8 ratios fell within the normal range except for one case, which showed a reversal of CD4/CD8, and this patient perished. His T suppressor cells were increased and had several cycles of malignant flare-up. One case showed mycosis fungoides, converting to lymphoma. This study demonstrates that monitoring of the progress is possible and can detect downgrading of malignant lymphomas.
该研究利用针对T细胞和B细胞的单克隆抗体来研究我们淋巴瘤中的免疫表型，并将其与8例恶性淋巴瘤的临床病理特征相关联，其中4例为皮肤T细胞淋巴瘤，4例为恶性淋巴瘤。所采用的方法是通过碱性磷酸酶抗碱性磷酸酶（APAAP）程序对组织切片进行免疫组化染色。对于4例皮肤T细胞淋巴瘤，3例处于1期，1例处于2期。免疫表型为蕈样肉芽肿型皮肤T细胞淋巴瘤的典型表现。它们主要是T细胞，有许多成熟T细胞。CD4与CD8的比值在正常范围内。没有CD7的特异性缺失。对于4例T细胞型恶性淋巴瘤，其模式同样是具有CD2、CD5、CD7和CD4的T细胞表型。除1例CD4/CD8比值倒置的病例外，其余病例的CD4与CD8比值均在正常范围内，该患者死亡。其T抑制细胞增加，并有多个恶性发作周期。1例显示蕈样肉芽肿转变为淋巴瘤。这项研究表明对病情进展进行监测是可行的，并且能够检测到恶性淋巴瘤的病情恶化。
