Racchi M, Bergamaschi S, Govoni S, Wetsel W C, Bianchetti A, Binetti G, Battaini F, Trabucchi M
Institute of Pharmacological Sciences, University of Milan, Italy.
Arch Biochem Biophys. 1994 Oct;314(1):107-11. doi: 10.1006/abbi.1994.1417.
In the present study we have examined the distribution of several isoforms of protein kinase C, a lipid-regulated serine/threonine kinase essential for signal transduction and cell regulation, in cultured human skin fibroblasts. By Western blot analysis we have detected the presence of at least three of the known protein kinase C isoforms. The calcium-dependent protein kinase C alpha was primarily associated with the cytosolic fraction. Three non-calcium-dependent isoforms, protein kinases C epsilon, C delta, and C zeta, were also detected. Protein kinases C zeta and C delta were present primarily in the cytosol, while protein kinase C epsilon was associated primarily with the membrane fraction. Binding and activity studies were consistent with the pattern of expression and distribution defined by Western blot analysis. These results provide a useful frame of reference for the study of isoform-specific effects of protein kinase C in the regulation of cell growth and metabolism.
在本研究中，我们检测了蛋白激酶C的几种同工型在培养的人皮肤成纤维细胞中的分布情况。蛋白激酶C是一种脂质调节的丝氨酸/苏氨酸激酶，对信号转导和细胞调节至关重要。通过蛋白质印迹分析，我们检测到至少三种已知的蛋白激酶C同工型的存在。钙依赖性蛋白激酶Cα主要与胞质部分相关。还检测到三种非钙依赖性同工型，即蛋白激酶Cε、Cδ和Cζ。蛋白激酶Cζ和Cδ主要存在于胞质溶胶中，而蛋白激酶Cε主要与膜部分相关。结合和活性研究与蛋白质印迹分析所确定的表达和分布模式一致。这些结果为研究蛋白激酶C在细胞生长和代谢调节中的同工型特异性作用提供了有用的参考框架。
