Ganetzky B
Laboratory of Genetics, University of Wisconsin, Madison 53706.
Bioessays. 1994 Jul;16(7):461-3. doi: 10.1002/bies.950160704.
Multidisciplinary studies have led to the discovery and characterization of cysteine string proteins (csps) in both Drosophila and Torpedo. Phenotypic analysis of csp mutants in Drosophila demonstrates a crucial role for csp in synaptic transmission. Expression studies of Torpedo csp (Tcsp) in Xenopus oocytes suggests that the protein has some role in the function of presynaptic Ca2+ channels. However, biochemical purification of Tcsp indicates that is associated with synaptic vesicles rather than with the plasma membrane of presynaptic terminals where Ca2+ channels reside. These results suggest a model in which csps serve as a link by which docked synaptic vesicles could modulate the activity of presynaptic Ca2+ channels.
多学科研究已促成在果蝇和电鳐中发现并鉴定了半胱氨酸串珠蛋白（csps）。对果蝇中csp突变体的表型分析表明，csp在突触传递中起关键作用。在非洲爪蟾卵母细胞中对电鳐csp（Tcsp）进行的表达研究表明，该蛋白在突触前Ca2+通道的功能中发挥某种作用。然而，对Tcsp的生化纯化表明，它与突触小泡相关，而非与Ca2+通道所在的突触前终末的质膜相关。这些结果提示了一个模型，即csps作为一种连接物，停靠的突触小泡可借此调节突触前Ca2+通道的活性。
