牛内皮素转化酶的cDNA克隆与表达
cDNA cloning and expression of bovine endothelin converting enzyme.
作者信息
Ikura T, Sawamura T, Shiraki T, Hosokawa H, Kido T, Hoshikawa H, Shimada K, Tanzawa K, Kobayashi S, Miwa S
机构信息
Department of Pharmacology, Faculty of Medicine, Kyoto University, Japan.
出版信息
Biochem Biophys Res Commun. 1994 Sep 30;203(3):1417-22. doi: 10.1006/bbrc.1994.2343.
We have cloned cDNA encoding bovine endothelin converting enzyme (ECE). The predicted amino acid sequence of bovine ECE consisted of 758 amino acid residues. Northern blot analysis revealed that ECE mRNA was abundantly expressed in lung. Co-expression of the cloned cDNA of bovine ECE with human preproET-1 cDNA in CHO-K1 cells resulted in the production of mature ET-1 and this production was inhibited by phosphoramidon.
我们克隆了编码牛内皮素转换酶（ECE）的cDNA。牛ECE的预测氨基酸序列由758个氨基酸残基组成。Northern印迹分析显示，ECE mRNA在肺中大量表达。在CHO-K1细胞中，牛ECE的克隆cDNA与人前体大内皮素-1 cDNA共表达导致成熟内皮素-1的产生，并且这种产生受到磷酰胺素的抑制。
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