急性胰腺炎大鼠的血清可诱导胰腺腺泡细胞系AR-42J中PAP mRNA的表达。

Serum from rats with acute pancreatitis induces expression of the PAP mRNA in the pancreatic acinar cell line AR-42J.

作者信息

Dusetti N J, Mallo G, Dagorn J C, Iovanna J L

机构信息

U.315 INSERM, Marseille, France.

出版信息

Biochem Biophys Res Commun. 1994 Oct 14;204(1):238-43. doi: 10.1006/bbrc.1994.2450.

DOI:10.1006/bbrc.1994.2450

PMID:7945366

Abstract

PAP is a pancreatic secretory protein expressed in the pancreas during the acute phase of pancreatitis. We have investigated the effect of the serum from rats with acute pancreatitis (SAP) on the expression of the PAP mRNA in AR-42J cells. PAP mRNA is strongly induced by SAP in a dose-dependent manner. This induction is abolished by preheating the SAP or diminished by treating the cells with cycloheximide. In addition, amylase but not actin mRNA expression was induced by a different SAP factor. We transfected the AR-42J cells with a chimeric gene containing 1.2 kbp 5'-flanking region of the PAP promoter linked to the CAT reporter gene. The CAT activity was significatively increased in the cells, on treating them with SAP. Our results show: first, SAP contains factors responsible for the PAP mRNA expression and secondly, the cis-acting elements are localized within the 1.2 kbp upstream region of the transcription initiation site.

摘要

PAP是一种在胰腺炎急性期胰腺中表达的胰腺分泌蛋白。我们研究了急性胰腺炎（SAP）大鼠血清对AR-42J细胞中PAP mRNA表达的影响。PAP mRNA被SAP以剂量依赖性方式强烈诱导。通过预热SAP可消除这种诱导作用，或用环己酰亚胺处理细胞可使其减弱。此外，一种不同的SAP因子可诱导淀粉酶而非肌动蛋白mRNA的表达。我们用一个包含与CAT报告基因相连的PAP启动子1.2kbp 5'侧翼区域的嵌合基因转染AR-42J细胞。用SAP处理细胞后，CAT活性显著增加。我们的结果表明：第一，SAP含有负责PAP mRNA表达的因子；第二，顺式作用元件位于转录起始位点上游1.2kbp区域内。