Kawamata N, Miki T, Ohashi K, Suzuki K, Fukuda T, Hirosawa S, Aoki N
First Department of Internal Medicine, Tokyo Medical and Dental University, Japan.
Biochem Biophys Res Commun. 1994 Oct 14;204(1):366-74. doi: 10.1006/bbrc.1994.2468.
The BCL6 gene involved in the 3q27 translocation associated with B-cell lymphomas encodes a novel Cys2-His2 zinc finger protein. We generated a fusion protein of glutathione S-transferase and zinc finger domain of BCL6 to determine recognition sequences of BCL6 with polymerase chain reaction using random oligonucleotides of 26 bases as a ligand. A consensus of 14 nucleotides consisting of (T/A)NCTTTCNAGG(A/G)AT was identified in the recognition sequences. In a gel mobility shift assay, the probe containing the 14-nucleotide recognition sequence formed a complex with the fusion protein and nuclear proteins from Burkitt's cell lines overexpressing the BCL6 transcripts. The consensus sequence was protected from the digestion by nuclease in a DNase I footprinting assay. In conclusion, BCL6 may be involved in tumorigenesis by binding to the consensus sequences of the other genes.
与B细胞淋巴瘤相关的3q27易位所涉及的BCL6基因编码一种新型的Cys2-His2锌指蛋白。我们构建了谷胱甘肽S-转移酶与BCL6锌指结构域的融合蛋白，以使用26个碱基的随机寡核苷酸作为配体，通过聚合酶链反应来确定BCL6的识别序列。在识别序列中鉴定出了一个由(T/A)NCTTTCNAGG(A/G)AT组成的14个核苷酸的共有序列。在凝胶迁移率变动分析中，含有14个核苷酸识别序列的探针与来自过表达BCL6转录本的伯基特细胞系的融合蛋白和核蛋白形成了复合物。在DNA酶I足迹分析中，该共有序列受到核酸酶消化的保护。总之，BCL6可能通过与其他基因的共有序列结合而参与肿瘤发生。
