Kaufmann M, Wink M
Universität Heidelberg, Institut für Pharmazeutische Biologie, Bundesrepublik Deutschland.
Z Naturforsch C J Biosci. 1994 Sep-Oct;49(9-10):635-45. doi: 10.1515/znc-1994-9-1015.
Total DNA was extracted from 41 species (20 genera) of the subfamily Nepetoideae (family Labiatae). Using rbcL-specific primers, the rbcL gene wsa amplified by polymerase chain reaction (PCR) and sequenced directly. RbcL sequences were evaluated with character state (maximum parsimony; PAUP) and distance methods (neighbour-joining; MEGA). In agreement with classical systematics all taxa studied cluster within the Nepetoideae and are clearly distinguished from members of the subfamily Lamioideae. A number of distinctive clades are apparent within the Nepetoideae: I-Collinsonia, II-Lavandula, III-Agastache, Glechoma, IV-Satureja, Hyssopus, Dracocephalum, V-Nepeta, VI-Hormium, VII-Prunella, VIII-Melissa, Ocimum, IX-Monarda, Mentha, X-Origanum, Thymus, XI-Salvia, XII-Rosmarinus, and XIII-Perovskia. At least five main branches representing the clades I, II, III to VII, VIII, and IX to XIII respectively, can be distinguished within the Nepetoideae studied. They might be considered representing the tribes (according to Cantino, 1992) Elsholtzieae (I). Lavanduleae (II), and Mentheae (III-XIII). The tribe Mentheae needs to be subdivided into at least three main groups (clades III-VII, VIII and IX-XIII). Majorana hortensis which is often classified as Origanum hortensis does not cluster with Origanum and deserves a genetic status of its own.
从唇形科荆芥亚科的41个物种（20个属）中提取了总DNA。使用rbcL特异性引物，通过聚合酶链反应（PCR）扩增rbcL基因并直接测序。用特征状态法（最大简约法；PAUP）和距离法（邻接法；MEGA）评估rbcL序列。与经典分类学一致，所有研究的分类群都聚类在荆芥亚科内，并与野芝麻亚科的成员明显区分开来。在荆芥亚科内有一些明显的独特分支：I - 柯林斯草属，II - 薰衣草属，III - 藿香属、活血丹属，IV - 风轮菜属、牛膝草属、青兰属，V - 荆芥属，VI - 薄荷属，VII - 夏枯草属，VIII - 蜜蜂花属、罗勒属，IX - 美国薄荷属、薄荷属，X - 牛至属、百里香属，XI - 鼠尾草属，XII - 迷迭香属，以及XIII - 糙苏属。在所研究的荆芥亚科内，可以区分出至少五个主要分支，分别代表分支I、II、III至VII、VIII以及IX至XIII。它们可能被认为代表了（根据坎蒂诺，1992）香薷族（I）、薰衣草族（II）和薄荷族（III - XIII）。通常被归类为牛至属hortensis的马郁兰不与牛至属聚类，应给予其独立的分类地位。
