Fang Y Q, Zhao W X, Lin Q M
Third Institute of Oceanography, SOA, Xiamen, PRC.
Sci China B. 1994 Jul;37(7):842-50.
Using the electron microscopic technique and radioimmunoassay, the hormonal regulation of the oocyte development and maturation of the amphioxus has been investigated. The results indicate that the exogenous GnRH-A can stimulate the oocyte development and maturation as well as the spawning of the amphioxus. It has further been revealed by radioimmunoassay that GnRH-A can increase the context of sex steroid hormone, especially, estradiol-17 beta, by 1-3 times. The increase of estradiol-17 beta coordinates with the synthesis of oocyte into yolk substance. The results further justify that the hormonal regulation in the oocyte development and maturation of amphioxus is similar to that of vertebrates. This is of great theoretical significance for reproductive endocrine physiology of amphioxus.
运用电子显微镜技术和放射免疫分析法，对文昌鱼卵母细胞发育和成熟的激素调节进行了研究。结果表明，外源性促性腺激素释放激素类似物（GnRH-A）能刺激文昌鱼的卵母细胞发育、成熟以及产卵。放射免疫分析进一步显示，GnRH-A能使性类固醇激素，尤其是雌二醇-17β的含量增加1至3倍。雌二醇-17β的增加与卵母细胞合成卵黄物质相协调。这些结果进一步证明，文昌鱼卵母细胞发育和成熟过程中的激素调节与脊椎动物相似。这对文昌鱼的生殖内分泌生理学具有重要的理论意义。
