作为水合作用函数的铯DNA薄膜的光学性质。

Optical properties of CsDNA films as a function of hydration.

作者信息

Adams P J, VanSteenberg M L, Lee S A, Rupprecht A

机构信息

Department of Physics and Astronomy, University of Toledo, OH 43606.

出版信息

J Biomol Struct Dyn. 1994 Jun;11(6):1277-86. doi: 10.1080/07391102.1994.10508068.

DOI:10.1080/07391102.1994.10508068

PMID:7946074

Abstract

The refractive indices of wet-spun films of CsDNA have been measured for light polarized parallel and perpendicular to the helical axis as a function of relative humidity (RH). These data have been combined with previously published data (Biopolymers 30 (1990) 877-887) for the volume per base pair and water content as a function of RH in order to extract the optical polarizabilities. This work was motivated by the study of Weidlich et al. (Biopolymers 26 (1987) 439-453) who reported a approximately 35% increase at the A-to-B transition in the parallel and perpendicular polarizabilities of NaDNA. In contrast, a much smaller increase in the polarizabilities of CsDNA is found near the A-to-B transition: approximately 12% for the perpendicular direction and < or = 4% for the parallel direction.

摘要

已测量了铯脱氧核糖核酸（CsDNA）湿纺薄膜对于平行和垂直于螺旋轴偏振光的折射率，该折射率是相对湿度（RH）的函数。这些数据已与先前发表的数据（《生物聚合物》30（1990）877 - 887）相结合，后者给出了每碱基对的体积和水含量作为相对湿度的函数，以便提取光学极化率。这项工作是受魏德利希等人（《生物聚合物》26（1987）439 - 453）的研究启发，他们报告说在NaDNA的A到B转变中，平行和垂直极化率大约增加了35%。相比之下，在CsDNA的A到B转变附近，极化率的增加要小得多：垂直方向约为12%，平行方向≤4%。