果蝇肿瘤抑制基因dlg是正常突触小体结构所必需的。
The Drosophila tumor suppressor gene dlg is required for normal synaptic bouton structure.
作者信息
Lahey T, Gorczyca M, Jia X X, Budnik V
机构信息
Department of Biology, University of Massachusetts, Amherst 01003.
出版信息
Neuron. 1994 Oct;13(4):823-35. doi: 10.1016/0896-6273(94)90249-6.
Abstract
The Drosophila tumor suppressor gene lethal (1) discs large (dlg) encodes a protein necessary for normal cell growth in epithelial and brain tissue. It shares high sequence identity to the mammalian synaptic proteins PSD-95 and SAP-70, whose functions are unknown. To determine the localization and role of dlg at synapses, we investigated its distribution and the effects of dlg mutations on Drosophila neuromuscular junctions. We show that dlg immunoreactivity is expressed at one type of glutamatergic synapse and is associated with both presynaptic and postsynaptic membranes. Mutations in dlg alter the expression of dlg and cause striking changes in the structure of the subsynaptic reticulum, a postsynaptic specialization at these synapses. These results indicate that dlg is required for normal synaptic structure and offer insights regarding the role of dlg homologs at vertebrate synapses.
摘要
果蝇肿瘤抑制基因致死（1）大圆盘（dlg）编码上皮组织和脑组织中正常细胞生长所必需的一种蛋白质。它与哺乳动物突触蛋白PSD - 95和SAP - 70具有高度的序列同一性，而这两种蛋白的功能尚不清楚。为了确定dlg在突触中的定位和作用，我们研究了它在果蝇神经肌肉接头处的分布以及dlg突变的影响。我们发现dlg免疫反应性在一种谷氨酸能突触中表达，并与突触前膜和突触后膜相关联。dlg中的突变改变了dlg的表达，并导致突触下网状结构（这些突触处的一种突触后特化结构）的结构发生显著变化。这些结果表明，正常的突触结构需要dlg，并为dlg同源物在脊椎动物突触中的作用提供了见解。
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