Macdonald R, Xu Q, Barth K A, Mikkola I, Holder N, Fjose A, Krauss S, Wilson S W
Developmental Biology Research Centre, Randall Institute, Kings College London, England.
Neuron. 1994 Nov;13(5):1039-53. doi: 10.1016/0896-6273(94)90044-2.
During development of the zebrafish forebrain, a simple scaffold of axon pathways is pioneered by a small number of neurons. We show that boundaries of expression domains of members of the eph, forkhead, pax, and wnt gene families correlate with the positions at which these neurons differentiate and extend axons. Analysis of genetically or experimentally altered forebrains indicates that if a boundary is maintained, there is appropriate neural differentiation with respect to the boundary. Conversely, in the absence of a boundary, there is concomitant disruption of neural patterning. We also show that a strip of cells within the dorsal diencephalon shares features with ventral midline cells. This strip of cells fails to develop in mutant fish in which specification of the ventral CNS is disrupted, suggesting that its development may be regulated by the same inductive pathways that pattern the ventral midline.
在斑马鱼前脑发育过程中，少数神经元开创了一个简单的轴突通路支架。我们发现，eph、叉头框、pax和wnt基因家族成员的表达域边界与这些神经元分化和延伸轴突的位置相关。对基因改变或实验改变的前脑的分析表明，如果边界得以维持，相对于边界就会有适当的神经分化。相反，在没有边界的情况下，神经模式会同时受到破坏。我们还表明，背侧间脑内的一条细胞带与腹侧中线细胞具有共同特征。在腹侧中枢神经系统特化被破坏的突变鱼中，这条细胞带无法发育，这表明其发育可能受与腹侧中线模式化相同的诱导途径调控。
