Vahl C F, Bonz A, Hagl C, Hagl S
Department of Cardiac Surgery, University of Heidelberg, Germany.
Eur J Cardiothorac Surg. 1994;8(7):370-8. doi: 10.1016/1010-7940(94)90031-0.
The influence of 2,3-Butanedione monoxime (BDM) on the human myocardium's tolerance to cold ischemia was analyzed in two experimental series.
I) Left ventricular human muscle fibers (0.6 x 4.0 mm) were obtained from recipient hearts (n = 10) and loaded with the fluorescent dye Fura-2. Simultaneous measurements of intracellular calcium transients ("ratio-method"; excitation wave lengths: 340 nm and 380 nm) and isometric force development of electrically driven (1 Hz) muscle fibers were carried out at BDM concentrations ranging from 0 to 30 mM at a bath temperature of 37 degrees C; II) Left ventricular human muscle strips were obtained from beating recipient hearts (n = 10), and right atrial fibers from patients operated upon for aortic valve stenosis or combined mitral valve disease (n = 14). Muscle strips of these hearts were incubated for parallel measurements in the following solutions: a) a 37 degrees C oxygenated Krebs-Henseleit solution (KHS), b) a 4 degrees C Bretschneider's cardioplegic solution (HTK) without oxygenation and c) a 4 degrees C KHS containing 30 mM BDM without oxygenation (BDM solution). After standardized time intervals the muscle fibers were removed from the storage solutions, reperfused in KHS solution at 37 degrees C and stretched to optimal length (supramaximal electrical stimulation). After obtaining a steady state of force development, the contractile behavior under isometric and isotonic measurement conditions was measured. The influence of the incubation periods and the incubation solution was analyzed.
I) BDM reduced the isometric force development of the electrically driven isolated human myocardial muscle strip in a dose-dependent way.(ABSTRACT TRUNCATED AT 250 WORDS)
在两个实验系列中分析了2,3-丁二酮一肟（BDM）对人心肌冷缺血耐受性的影响。
I）从受体心脏（n = 10）获取左心室人体肌纤维（0.6×4.0毫米），并用荧光染料Fura-2加载。在37℃的浴温下，在BDM浓度范围为0至30 mM时，同时测量细胞内钙瞬变（“比率法”；激发波长：340 nm和380 nm）以及电驱动（1 Hz）肌纤维的等长力发展；II）从跳动的受体心脏（n = 10）获取左心室人体肌条，从接受主动脉瓣狭窄或二尖瓣联合疾病手术的患者（n = 14）获取右心房纤维。将这些心脏的肌条在以下溶液中进行平行孵育：a）37℃的含氧Krebs-Henseleit溶液（KHS），b）4℃的无氧Bretschneider心脏停搏液（HTK），c）4℃的含30 mM BDM的无氧KHS（BDM溶液）。在标准化时间间隔后，将肌纤维从储存溶液中取出，在37℃的KHS溶液中再灌注并拉伸至最佳长度（超最大电刺激）。在获得力发展的稳定状态后，测量等长和等张测量条件下的收缩行为。分析孵育时间和孵育溶液的影响。
I）BDM以剂量依赖性方式降低了电驱动的离体人心肌肌条的等长力发展。（摘要截断于250字）
