De Grooth R, Mieremet R H, Kawilarang-De Haas E W, Nijweide P J
Department of Cell Biology, University of Leiden, The Netherlands.
Int J Exp Pathol. 1994 Aug;75(4):265-75.
Six murine macrophage precursor cell lines, thought to be arrested around the CFU-GM stage of the myeloid differentiation and shown to be negative for acid phosphatase, F4/80 antigen expression and phagocytosis capacity, were tested for their ability to differentiate into osteoclasts. Their differentiation potential was compared with that of the haemopoietic stem cell line FDCP-mix C2GM. None of the macrophage precursor cell lines could be induced to differentiate into osteoclasts when the cells were cocultured with either periosteum-free metatarsal bones of fetal mice, or monolayers of osteoblast-like cells. In contrast, when the haemopoietic stem cell line FDCP-mix C2GM, murine fetal liver cells or murine spleen cells were used as a source of haemopoietic precursor cells, numerous osteoclasts were formed in both culture systems. During cell culture a small percentage of the macrophage precursor cells attached to the bottom of the culture well. These firmly attached cells acquired acid phosphatase activity, F4/80 antigen expression and phagocytosis capacity. Furthermore, when the cell lines were cultured for 2 or 4 days with 1% DMSO, up to 30% of the precursor cells differentiated into metamyelocytes. These results suggest that the macrophage precursor cell lines are able to acquire macrophage and granulocyte characteristics, but are unable to differentiate into osteoclasts. In contrast, the haemopoietic stem cell line FDCP-mix C2GM is able to differentiate into both macrophages and osteoclasts. We therefore suggest that the osteoclast lineage branches off at an early stage of the myeloid differentiation pathway.
六个小鼠巨噬细胞前体细胞系，被认为在髓系分化的CFU - GM阶段停滞，且已证明其酸性磷酸酶、F4/80抗原表达和吞噬能力均为阴性，对它们分化为破骨细胞的能力进行了测试。将它们的分化潜能与造血干细胞系FDCP - mix C2GM的分化潜能进行了比较。当这些巨噬细胞前体细胞系与无骨膜的胎鼠跖骨或成骨细胞样细胞单层共培养时，没有一个能被诱导分化为破骨细胞。相比之下，当使用造血干细胞系FDCP - mix C2GM、小鼠胎儿肝细胞或小鼠脾细胞作为造血前体细胞来源时，在两种培养系统中都形成了大量破骨细胞。在细胞培养过程中，一小部分巨噬细胞前体细胞附着在培养孔底部。这些牢固附着的细胞获得了酸性磷酸酶活性、F4/80抗原表达和吞噬能力。此外，当细胞系用1%二甲基亚砜培养2天或4天时，高达30%的前体细胞分化为晚幼粒细胞。这些结果表明，巨噬细胞前体细胞系能够获得巨噬细胞和粒细胞特征，但不能分化为破骨细胞。相比之下，造血干细胞系FDCP - mix C2GM能够分化为巨噬细胞和破骨细胞。因此，我们认为破骨细胞谱系在髓系分化途径的早期阶段分支出来。
