Geurts van Kessel A H, Tetteroo P A, von dem Borne A E, Hagemeijer A, Bootsma D
Proc Natl Acad Sci U S A. 1983 Jun;80(12):3748-52. doi: 10.1073/pnas.80.12.3748.
Hybrid cell lines were obtained after fusion of mouse myeloid cells (WEHI-TG) with leukocytes from two patients with chronic myeloid leukemia. A third fusion was carried out with leukocytes from a patient with acute lymphocytic leukemia. All three patients carried the Philadelphia chromosome (Ph1) in the leukemia cell population. Cytochemical analysis confirmed the myelo-monocytic nature of the hybrid cell lines. The presence of Ph1 translocation products could be established in most hybrids derived from the two chronic myeloid leukemic patients, which confirms that indeed human myeloid cells were fused. Several of these hybrid lines showed reactivity with monoclonal antibodies known to be specific for human myeloid cells, whereas interlineage Chinese hamster fibroblast-human chronic myeloid leukemia hybrids failed to react with these antibodies. Five independently obtained monoclonal antibodies--MI/NI, UJ-308, VIM-D5, FMC-10, and B4.3--showed very similar reactivity patterns when tested on the hybrid clones. This result substantiates the evidence obtained from other studies, that these five antibodies are directed against the same myeloid-associated antigen. The gene(s) for expression of the latter antigen could be assigned to human chromosome 11.
小鼠髓样细胞（WEHI-TG）与两名慢性髓性白血病患者的白细胞融合后获得了杂交细胞系。与一名急性淋巴细胞白血病患者的白细胞进行了第三次融合。所有三名患者的白血病细胞群体中都携带费城染色体（Ph1）。细胞化学分析证实了杂交细胞系的髓单核细胞性质。在源自两名慢性髓性白血病患者的大多数杂交细胞中都能检测到Ph1易位产物的存在，这证实了确实发生了人髓样细胞的融合。其中一些杂交细胞系与已知对人髓样细胞具有特异性的单克隆抗体发生反应，而种间中国仓鼠成纤维细胞-人慢性髓性白血病杂交细胞则不与这些抗体发生反应。当对杂交克隆进行检测时，五种独立获得的单克隆抗体——MI/NI、UJ-308、VIM-D5、FMC-10和B4.3——表现出非常相似的反应模式。这一结果证实了其他研究获得的证据，即这五种抗体针对的是同一种髓样相关抗原。后一种抗原的表达基因可定位于人类11号染色体。
