Nakamura J
Institute of Clinical Medicine, University of Tsukuba, Ibaraki-ken, Japan.
Biochim Biophys Acta. 1994 Nov 16;1209(1):111-6. doi: 10.1016/0167-4838(94)90145-7.
The activity and the amount of Gs in wild-type S49 murine lymphoma cells decreased by 16-18% and by 29%, respectively, after 24 h treatment of the cells with 50 mM ethanol, through a transient increase of the Gs activity with no alteration in its content after 4 and 8 h treatment. No decrease of the Gs activity was noted when membranes from S49 cells were treated with 50-500 mM ethanol. To study the role of protein metabolism in the ethanol-induced reduction in the amount of Gs, S49 cells were treated with ethanol for 24 h in the presence of inhibitor of protein synthesis. Cycloheximide at concentrations of 0.05-0.15 micrograms/ml and puromycin at 0.15-0.60 micrograms/ml caused a dose-dependent inhibition of cell division in S49 cells to a similar degree. The ethanol-induced reduction of the activity and the amount of Gs was completely abolished by those concentrations of cycloheximide, but not by puromycin. Cycloheximide itself caused no alteration in the Gs activity of S49 cell membranes. These results indicate that the process of the ethanol-induced event of Gs involves cycloheximide-sensitive protein synthesis.
用50 mM乙醇处理野生型S49鼠淋巴瘤细胞24小时后，Gs的活性和含量分别下降了16 - 18%和29%，在处理4小时和8小时后，Gs活性短暂增加，但其含量未改变。用50 - 500 mM乙醇处理S49细胞膜时，未观察到Gs活性降低。为了研究蛋白质代谢在乙醇诱导的Gs含量降低中的作用，在存在蛋白质合成抑制剂的情况下，用乙醇处理S49细胞24小时。浓度为0.05 - 0.15微克/毫升的环己酰亚胺和0.15 - 0.60微克/毫升的嘌呤霉素对S49细胞的细胞分裂产生了相似程度的剂量依赖性抑制。这些浓度的环己酰亚胺完全消除了乙醇诱导的Gs活性和含量的降低，但嘌呤霉素没有。环己酰亚胺本身对S49细胞膜的Gs活性没有影响。这些结果表明，乙醇诱导的Gs事件过程涉及对环己酰亚胺敏感的蛋白质合成。
