Baase W A, Johnson W C
Nucleic Acids Res. 1976 Nov;3(11):3123-31. doi: 10.1093/nar/3.11.3123.
The circular dichroism of membrane-free folded chromosomes from E. coli was measured and analyzed. The spectrum can be explained as a simple linear combination of the individual spectra of E. coli RNA, and E. Coli DNA in the B form. No contribution from A form or C form DNA was detected. There was evidently some real variation in the ratio of the two nucleic acids from preparation to preparation, but the average value was 24% RNA and 76% DNA. No significant light scattering was observed and the analyses indicated no contribution to the circular dichroism from scattering artifacts. Apparently, combining DNA, RNA, and protein into membrane-free folded chromosomes does not change the secondary structure of the DNA or RNA from that found for the free nucleic acid in the same solvent system.
对来自大肠杆菌的无膜折叠染色体的圆二色性进行了测量和分析。该光谱可以解释为大肠杆菌RNA和B型大肠杆菌DNA各自光谱的简单线性组合。未检测到A型或C型DNA的贡献。不同制备物中两种核酸的比例显然存在一些实际差异，但平均值为24%的RNA和76%的DNA。未观察到明显的光散射，分析表明散射伪像对圆二色性没有贡献。显然，将DNA、RNA和蛋白质组合成无膜折叠染色体不会改变DNA或RNA的二级结构，与在相同溶剂系统中游离核酸的二级结构相同。
