Braga M, Di Francesco A, Gianotti L, Vignali A, Costantini E, Socci C, Fortis C, Paganelli G, Di Carlo V
Department of Surgery, Scientific Institute S. Raffaele Hospital, University of Milan, Italy.
Eur J Surg. 1994 Jun-Jul;160(6-7):345-50.
To investigate the effect of blood transfusion on mortality and the incidence of bacterial translocation in mice subjected to thermal burn or bacterial gavage, or both, and to assess the influence of thymopentin on mortality.
Randomly controlled experiments.
University departments of surgery, immunology and nuclear medicine.
235 Balb/c (H-2d) and C3H/HeJ (H-2k) mice.
8 groups of 20 mice each received: saline infusion (controls), blood transfusion (BT) alone, 20% burn alone, gavage with 1 x 10(10) Escherichia coli alone, BT and gavage, BT and burn, burn and gavage, or BT, burn, and gavage. A further 3 groups of 10 mice were all gavaged with 111In-biotin labelled E coli and randomised to additional BT and burn, BT alone, or burn alone. 98 mice that had had BT, burn, and gavage, were then randomised to receive thymopentin 0, 0.1, 1, or 5 mg/kg/day for 15 days. The impact of the pretreatment with thymopentin on PGE2 concentration was also evaluated in a separate group of 45 mice that received BT, burn, and gavage; or burn and gavage.
Survival, degree of translocation.
The highest mortality (75%) was in the BT, burn, and gavage group. BT alone significantly reduced survival in burned mice, whereas BT alone or associated with gavage had no effect. Thermal injury had the most influence on bacterial translocation, whereas BT did not increase it. Thymopentin significantly improved survival, particularly in the higher doses. The pretreatment with thymopentin significantly reduced PGE2 concentration after BT, burn and gavage.
Burn injury significantly increased mortality in the presence of immune deficiency caused by BT. Thymopentin reduced mortality, possibly by immunomodulation.
研究输血对热烧伤或经口给予细菌或二者兼有的小鼠死亡率及细菌易位发生率的影响，并评估胸腺五肽对死亡率的影响。
随机对照实验。
大学外科、免疫学和核医学系。
235只Balb/c（H-2d）和C3H/HeJ（H-2k）小鼠。
8组，每组20只小鼠，分别接受：生理盐水输注（对照组）、单纯输血（BT）、单纯20%烧伤、单纯经口给予1×10¹⁰大肠杆菌、输血加经口给予细菌、输血加烧伤、烧伤加经口给予细菌、或输血、烧伤及经口给予细菌。另外3组，每组10只小鼠均经口给予¹¹¹In-生物素标记的大肠杆菌，并随机分为额外输血加烧伤、单纯输血、或单纯烧伤组。然后，将98只接受了输血、烧伤及经口给予细菌的小鼠随机分为接受胸腺五肽0、0.1、1或5mg/kg/天，共15天。还在另一组45只接受输血、烧伤及经口给予细菌；或烧伤及经口给予细菌的小鼠中评估胸腺五肽预处理对前列腺素E2浓度的影响。
生存率、易位程度。
输血、烧伤及经口给予细菌组的死亡率最高（75%）。单纯输血显著降低烧伤小鼠的生存率，而单纯输血或输血加经口给予细菌则无影响。热损伤对细菌易位影响最大，而输血并未增加细菌易位。胸腺五肽显著提高生存率，尤其是高剂量时。胸腺五肽预处理显著降低输血、烧伤及经口给予细菌后的前列腺素E2浓度。
在输血导致的免疫缺陷情况下，烧伤显著增加死亡率。胸腺五肽可能通过免疫调节降低死亡率。
