Zhuang W, Sugimoto C, Matsuba T, Niinuma S, Murata M, Onuma M
Department of Epizootiology, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
J Vet Med Sci. 1994 Jun;56(3):469-73. doi: 10.1292/jvms.56.469.
Monoclonal antibodies (MoAbs) against Theileria sergenti Fukushima stock, an intraerythrocytic protozoan parasite of cattle, were produced. The MoAbs reacted with 32 kDa or 23 kDa piroplasm surface protein (p32 or p23) in Western blot analysis. Using a panel of the MoAbs, antigenic analysis of the stocks and field isolates distributed in Japan was carried out. The results revealed antigenic diversity of T. sergenti isolates, but diversity did not appear to be correlated with the geographical region of the isolates. N-terminal amino acids sequences analysis revealed substitutions of a few amino acids between the p23 of two T. sergenti stocks. The sequences couldn't be found in amino acid sequence of the p32 deduced from cDNA, which indicated that the p23 is not a proteolytic product of the p32. Genetic diversity of T. sergenti isolates was also observed by Southern blot analysis using a cDNA of the p32 as a probe.
制备了针对牛红细胞内原虫寄生虫瑟氏泰勒虫福岛株的单克隆抗体（MoAb）。在蛋白质免疫印迹分析中，这些单克隆抗体与32 kDa或23 kDa梨形虫表面蛋白（p32或p23）发生反应。使用一组单克隆抗体，对分布于日本的毒株和野外分离株进行了抗原分析。结果揭示了瑟氏泰勒虫分离株的抗原多样性，但这种多样性似乎与分离株的地理区域无关。N端氨基酸序列分析显示，两种瑟氏泰勒虫毒株的p23之间有几个氨基酸发生了替换。在从cDNA推导的p32氨基酸序列中未发现这些序列，这表明p23不是p32的蛋白水解产物。使用p32的cDNA作为探针，通过Southern印迹分析也观察到了瑟氏泰勒虫分离株的遗传多样性。
