Yildirim O, Akbulut U, Arinç E, Sungur S
Department of Biology, Ankara University, Sciences Faculty, Turkey.
Biomaterials. 1994 Jun;15(8):587-92. doi: 10.1016/0142-9612(94)90208-9.
NADH-cytochrome b5 reductase was isolated and partially purified from rabbit liver microsomes. It was immobilized into gelatin by chemical cross-linking. Chromium (III) acetate was used as cross-linker. The effects of pH and temperature on the immobilized cytochrome b5 reductase were investigated. The reusability and storage stability of immobilized enzyme were also tested. Immobilized NADH-cytochrome b5 reductase activities were found to be stable for at least 72 d and 24 uses. The storage stability of NADH-cytochrome b5 reductase was improved with immobilization at 25 degrees C.
从兔肝微粒体中分离并部分纯化了NADH-细胞色素b5还原酶。通过化学交联将其固定在明胶中。使用醋酸铬（III）作为交联剂。研究了pH值和温度对固定化细胞色素b5还原酶的影响。还测试了固定化酶的可重复使用性和储存稳定性。发现固定化的NADH-细胞色素b5还原酶活性在至少72天和24次使用中保持稳定。在25℃下固定化提高了NADH-细胞色素b5还原酶的储存稳定性。
