来自猪蛔虫（线虫纲）的铜锌超氧化物歧化酶：纯化与特性鉴定

Copper-zinc superoxide dismutase from Ascaris suum (Nematoda): purification and characterization.

作者信息

Sanchez-Moreno M, Entrala E, Janssen D, Osuna A

机构信息

Department of Biochemistry and Molecular Biology Faculty of Sciences, University of Granada, Spain.

出版信息

Biosci Rep. 1994 Apr;14(2):83-90. doi: 10.1007/BF01210303.

DOI:10.1007/BF01210303

PMID:7948773

Abstract

Copper-zinc superoxide dismutase from Ascaris suum (Nematoda) was purified in a new, more efficient, and faster manner. The process included differential centrifugation, fractionation with ammonium sulfate, and sodium dodecyl sulfate-polyacrylamide electrophoresis, yielding a 340-fold purification (specific activity of 47 units/mg). Optimal storage conditions, optimal pH range, thermostability, molecular weight and ultraviolet-visible absorption spectrum of the enzyme are described, and a new enzymatic model for pharmacological screening is suggested.

摘要

来自猪蛔虫（线虫纲）的铜锌超氧化物歧化酶以一种全新、更高效且更快的方式得到了纯化。该过程包括差速离心、硫酸铵分级分离以及十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳，得到了340倍的纯化效果（比活性为47单位/毫克）。文中描述了该酶的最佳储存条件、最佳pH范围、热稳定性、分子量以及紫外 - 可见吸收光谱，并提出了一种用于药理筛选的新酶学模型。