Schubert R, Panitz R, Manteuffel R, Nagy I, Wobus U, Bäumlein H
Institute of Plant Genetics and Crop Plant Research, Gatersleben, Germany.
Plant Mol Biol. 1994 Oct;26(1):203-10. doi: 10.1007/BF00039532.
We studied the expression of the oat globulin gene asglo5 in developing transgenic tobacco seeds. The asglo5 gene promoter directed transcription in the endosperm as well as in the provascular tissue, the presumptive root tip and the shoot apical meristem of the embryo as revealed by GUS reporter gene constructs and in situ hybridization. However, immunological tissue printing detected the oat protein exclusively in the tobacco endosperm, suggesting that extensive post-transcriptional regulatory processes influence the expression of the monocot transgene in the dicot host.
我们研究了燕麦球蛋白基因asglo5在转基因烟草种子发育过程中的表达。GUS报告基因构建体和原位杂交显示，asglo5基因启动子在胚乳以及原维管组织、胚的推测根尖和茎尖分生组织中指导转录。然而，免疫组织印迹仅在烟草胚乳中检测到燕麦蛋白，这表明广泛的转录后调控过程影响了单子叶转基因在双子叶宿主中的表达。
