Rivier A, Chanez P, Pène J, Michel F B, Godard P, Dugas B, Bousquet J
CJF INSERM 92-10, Hôpital Arnaud-de-Villeneuve, Montpellier, France.
Int Arch Allergy Immunol. 1994 May;104(1):27-32. doi: 10.1159/000236705.
In asthma, alveolar macrophages (AMs) are hyperreactive releasing large amounts of mediators and expressing high levels of surface markers. Granulocyte-macrophage colony-stimulating factor (GM-CSF) upregulates monocytes and AMs, and may be involved in the hyperreactivity of AMs. The effects of GM-CSF were tested on monocytes and AMs from normal subjects by examining the expression of factors thought to be upregulated in asthma. After various incubation times of GM-CSF, the expression of CD23 and beta 2-integrins (CD11a, CD11b, CD11c) was studied by FACS and the release of sCD23 was measured by ELISA. The priming and stimulatory effects of GM-CSF were tested on monocytes and AMs and the release of leukotriene B4 (LTB4) was measured by ELISA. GM-CSF induced the expression of CD11c and CD23 and the release of sCD23. GM-CSF primed and stimulated monocytes and AMs to release LTB4. The effects of GM-CSF may explain partly the hyperreactivity of AMs in asthma.
在哮喘中，肺泡巨噬细胞（AMs）反应性过高，会释放大量介质并表达高水平的表面标志物。粒细胞-巨噬细胞集落刺激因子（GM-CSF）可上调单核细胞和AMs，可能参与AMs的高反应性。通过检测哮喘中被认为上调的因子的表达，来测试GM-CSF对正常受试者单核细胞和AMs的作用。在GM-CSF进行不同孵育时间后，通过流式细胞术研究CD23和β2整合素（CD11a、CD11b、CD11c）的表达，并通过酶联免疫吸附测定法测量可溶性CD23（sCD23）的释放。测试GM-CSF对单核细胞和AMs的启动和刺激作用，并通过酶联免疫吸附测定法测量白三烯B4（LTB4）的释放。GM-CSF诱导CD11c和CD23的表达以及sCD23的释放。GM-CSF启动并刺激单核细胞和AMs释放LTB4。GM-CSF的作用可能部分解释了哮喘中AMs的高反应性。
