Mongeau R, Brassard R
Banting Research Centre, Nutrition Research Division, Ottawa, ON, Canada.
J AOAC Int. 1994 Sep-Oct;77(5):1197-202.
Dried beans (kidney and great northern) and dried peas (chick, green, and yellow) were cooked according to package instructions. Total dietary fiber (TDF) was measured by the Mongeau (AOAC 992.16), Prosky (AOAC 985.29), and Lee (AOAC 991.43) methods (A, B, and C, respectively). Nonstarch polysaccharides (NSP) were measured by the Englyst gas-chromatographic method that included dimethyl sulfoxide treatment, and the lignin measured separately was added to NSP (method D). TDF was also measured by the Li method (E). TDF values ranged from 7 to 48 g/100 g dry weight. Methods B and C gave similar TDF values, which were higher than those from other methods. For kidney beans I, TDF values by methods B and C were up to 2.5 times higher because of the inclusion of starch. The fiber residues from methods A and C contained the same amounts of arabinose, xylose, mannose, galactose, and uronic acid, but glucose was 4 times higher in residue C than in residue A. When alpha-amylase from porcine pancreas was incorporated in methods B or C for 5 samples, the discrepancies among methods A, B, and C were reduced by 60-98%.
干豆（红腰豆和大白芸豆）和干豌豆（鹰嘴豆、青豌豆和黄豌豆）按照包装说明进行烹饪。总膳食纤维（TDF）采用蒙乔（AOAC 992.16）、普罗斯基（AOAC 985.29）和李（AOAC 991.43）方法进行测定（分别为方法A、B和C）。非淀粉多糖（NSP）采用恩格利斯特气相色谱法测定，该方法包括二甲基亚砜处理，单独测定的木质素加入到NSP中（方法D）。TDF也采用李方法进行测定（方法E）。TDF值范围为7至48克/100克干重。方法B和C得出的TDF值相似，且高于其他方法得出的值。对于红腰豆I，由于包含淀粉，方法B和C得出的TDF值高达其他方法得出值的2.5倍。方法A和C的纤维残渣中阿拉伯糖、木糖、甘露糖、半乳糖和糖醛酸的含量相同，但残渣C中的葡萄糖含量比残渣A中的高4倍。当将猪胰腺α淀粉酶加入方法B或C中用于5个样品时，方法A、B和C之间的差异减少了60 - 98%。
