Oishi M, Onishi K, Kano I, Nakazawa H, Tanabe S
Tama Branch Laboratory, Tokyo Metropolitan Research Laboratory of Public Health, Japan.
J AOAC Int. 1994 Sep-Oct;77(5):1293-6.
A rapid and simple procedure for the determination of thiabendazole (TBZ) residue in citrus and apple juices is described. A juice sample is made basic with 2M NaOH and applied to a disposable Extrelut prepacked column. TBZ is eluted with hexane-ethyl acetate (3 + 1) from the column. The eluate is evaporated to dryness under reduced pressure and then dissolved in an internal standard solution. TBZ is monitored without derivatization by capillary gas chromatography with nitrogen-phosphorus detection. The recoveries of TBZ added to fruit juices at 0.05-1.0 micrograms/g were 90-96%. The limit of detection of the method for TBZ was 0.01 micrograms/g. The proposed method is rapid, simple, and sensitive and is applicable to the determination of TBZ in commercial fruit juices.
描述了一种快速简便的测定柑橘汁和苹果汁中噻菌灵（TBZ）残留量的方法。用2M氢氧化钠将果汁样品碱化，然后加到一次性预填充的Extrelut柱上。用正己烷 - 乙酸乙酯（3 + 1）从柱上洗脱TBZ。洗脱液在减压下蒸发至干，然后溶解于内标溶液中。采用氮磷检测的毛细管气相色谱法，无需衍生化即可监测TBZ。向果汁中添加0.05 - 1.0微克/克的TBZ时，回收率为90 - 96%。该方法对TBZ的检测限为0.01微克/克。所提出的方法快速、简便且灵敏，适用于商业果汁中TBZ的测定。
