Harris D T, Schumacher M J, LoCascio J, Booth A, Bard J, Boyse E A
Department of Microbiology and Immunology, University of Arizona, Tucson.
Bone Marrow Transplant. 1994 Jul;14(1):63-8.
Recent clinical reports have demonstrated that umbilical cord blood (CB) may be utilized as a source of transplantable hematopoietic stem cells when bone marrow is not available. However, it is not apparent that CB can be used to transplant partially HLA-matched siblings or matched, non-familial recipients. In this study the immunoreactivity of CB has been investigated within familial confines; 14 families were analyzed at the time of birth of their child and six of these families were reassessed at 6 months post-partum. In mixed lymphocyte reactions, CB was unable to significantly respond to stimulation with cells from either the mother or father. Furthermore, unlike adult peripheral blood, CB displayed depressed immune responses to alloantigen and T cell mitogen. At 6 months, the immune responses of the infant demonstrated normal development in terms of alloantigen and mitogen responses. However, at 6 months both the mother and the infant demonstrated a continued immune tolerance to one another. The data suggest that CB could be used in familial transplant situations when siblings are HLA-haploidentical if the donor/recipients are chosen based on the paternal haplotype. Furthermore, maternal bone marrow harvested during the 6 months immediately following delivery of a child also should be suitable as a stem cell graft in haploidentical situations.
近期临床报告表明，在无法获取骨髓时，脐带血（CB）可作为可移植造血干细胞的来源。然而，CB能否用于移植部分HLA匹配的同胞或匹配的非家族性受者尚不清楚。在本研究中，在家族范围内对CB的免疫反应性进行了研究；在14个家庭的孩子出生时进行了分析，其中6个家庭在产后6个月进行了重新评估。在混合淋巴细胞反应中，CB对来自母亲或父亲的细胞刺激均无明显反应。此外，与成人外周血不同，CB对同种异体抗原和T细胞有丝分裂原的免疫反应较低。在6个月时，婴儿的免疫反应在同种异体抗原和有丝分裂原反应方面显示出正常发育。然而，在6个月时，母亲和婴儿彼此之间仍表现出持续的免疫耐受。数据表明，如果根据父系单倍型选择供体/受者，当同胞为HLA单倍型相同时，CB可用于家族性移植情况。此外，在孩子出生后立即的6个月内采集的母亲骨髓也应适合作为单倍型相同情况下的干细胞移植物。
