Kawabe H, Lopes O U, Chernicky C L, Brosnihan K B, Saruta T, Ferrario C M
Department of Internal Medicine, School of Medicine, Keio University, Tokyo, Japan.
Endocr J. 1993 Apr;40(2):249-56. doi: 10.1507/endocrj.40.249.
To further elucidate the role of the preoptic-hypothalamic region in fluid and electrolyte balance we studied the effect of surgical preoptic-hypothalamic disconnection using either a large (preoptic-hypothalamic disconnection) or a small (medial preoptic-hypothalamic disconnection) microknife. Both the large and small cuts seemed to transect the posterior projection originating in the periventricular tissue surrounding the anteroventral third ventricle (AV3V) and extending to supraoptic nucleus, but the supraoptic-neurohypophysial pathway was severed only by the large cut. Seven-day metabolic studies showed a disruption in hydromineral balance only in large cut rats; they had increased water intake and urine volume on day 1, a near-recovery of function on days 2 and 3, and polydipsia and polyuria on days 4 to 7. There was no difference between small cut rats and sham-operated rats in metabolic measurements. The large cut rats also had sustained hypernatremia and hyperosmolality, which was enhanced after water restriction for 48 h but was not accompanied by an increase in plasma arginine vasopressin. Our data therefore suggest that the efferent fibers running caudally from the AV3V are not involved in mediation of the hydromineral regulation of the AV3V.
为了进一步阐明视前区 - 下丘脑区域在体液和电解质平衡中的作用，我们使用大型（视前区 - 下丘脑切断术）或小型（内侧视前区 - 下丘脑切断术）显微刀研究了手术切断视前区 - 下丘脑的效果。大型和小型切口似乎都切断了起源于围绕前腹侧第三脑室（AV3V）的室周组织并延伸至视上核的后向投射，但只有大型切口切断了视上核 - 神经垂体通路。为期7天的代谢研究表明，只有大型切口的大鼠的水盐平衡受到破坏；它们在第1天饮水量和尿量增加，在第2天和第3天功能接近恢复，在第4天至第7天出现烦渴和多尿。小型切口大鼠和假手术大鼠在代谢测量方面没有差异。大型切口的大鼠还持续存在高钠血症和高渗状态，在限水48小时后加重，但血浆精氨酸加压素没有增加。因此，我们的数据表明，从AV3V尾侧发出的传出纤维不参与AV3V水盐调节的介导。
