Holeman J A, Danielson N D
Department of Chemistry, Miami University, Oxford, OH 45056.
J Chromatogr A. 1994 Sep 23;679(2):277-84. doi: 10.1016/0021-9673(94)80570-9.
The separation and detection of five antihistamine drugs commonly found within over-the-counter allergy and cold pharmaceutical products was performed by HPLC with chemiluminescence (CL) detection. Comparable detection limits at 5-10 pmol were found for the antihistamines by both UV at 214 nm and tris(2,2'-bipyridine) ruthenium(III) CL. However, urine samples were found not to generate as large an unretained peak by CL detection as compared to those peaks by UV detection at 214 and 254 nm. For example, the pheniramine peak representing 0.15 microgram/ml was almost totally obscured at 214 nm. Quantitative results received for three antihistamine commercial samples ranged from 4 to 8% error in accuracy when an internal standard was used to compensate for short term detector drift.
采用高效液相色谱法（HPLC）结合化学发光（CL）检测，对非处方抗过敏和感冒药中常见的五种抗组胺药进行分离和检测。通过214nm紫外检测和三（2,2'-联吡啶）钌（III）化学发光检测，发现抗组胺药的检测限在5-10皮摩尔之间相当。然而，与214nm和254nm紫外检测相比，尿液样品经化学发光检测产生的未保留峰不如紫外检测的峰大。例如，浓度为0.15微克/毫升的非尼拉敏峰在214nm处几乎完全被掩盖。当使用内标物来补偿短期检测器漂移时，三种抗组胺药商业样品的定量结果准确性误差在4%至8%之间。
