Matsubara N, Terabe S
Faculty of Science, Himeji Institute of Technology, Hyogo, Japan.
J Chromatogr A. 1994 Sep 30;680(1):311-5. doi: 10.1016/0021-9673(94)80081-2.
The separation of 24 dansylamino acids was investigated by capillary electrophoresis with an additive of micelles of a non-ionic surfactant, Tween 20. Although two pairs of peaks, norvaline and methionine derivatives, and didansyltyrosine and solvent (methanol), did not show good resolution, other dansylamino acids were well separated within 70 min using 100 mM Tween 20 and pH 2.40. The theoretical plate numbers calculated for dansylamino acids were 28,000-111,000 with a 19-cm capillary column.
采用非离子表面活性剂吐温20的胶束添加剂，通过毛细管电泳研究了24种丹磺酰氨基酸的分离情况。尽管正缬氨酸和甲硫氨酸衍生物、双丹磺酰酪氨酸和溶剂（甲醇）这两对峰没有表现出良好的分离效果，但使用100 mM吐温20和pH 2.40，其他丹磺酰氨基酸在70分钟内得到了很好的分离。使用19厘米的毛细管柱，计算得到的丹磺酰氨基酸的理论塔板数为28,000 - 111,000。
